Human Matriptase/ST14 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human Matriptase/ST14 Affinity Purified Polyclonal Ab (25 UG)

货号： AF3946-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF3946-SP
别名	EC 3.4.21; Epithin; HAI; Matriptase; Membrane-type serine protease 1; MTSP1; MT-SP1EC 3.4.21.109; prostamin; PRSS14; Serine protease 14; Serine protease TADG-15; SNC19; SNC19MTSP1; ST14; suppression of tumorigenicity 14 (colon carcinoma); suppression of tumorigenicity 14 (colon carcinoma, matriptase, epithin); suppressor of tumorigenicity 14 protein; TADG15; TADG-15; TMPRSS14; tumor associated differentially expressed gene 15 protein; Tumor-associated differentially-expressed gene 15 protein
反应种属	Human
应用	Western Blot(0.1 µg/mL) Intracellular Staining by Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects human Matriptase/ST14 Catalytic Domain in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant mouse Matriptase/Epithin is observed.
使用方法	Western Blot: 0.1 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/10⁶cells
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6768 (Human); 19143 (Mouse); 114093 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Matriptase promotes inflammatory cell accumulation and progression of established epidermal tumors. Authors: Sales K, Friis S, Abusleme L, Moutsopoulos N, Bugge T Oncogene, 2015;34(35):4664-72. Species: Mouse Sample Type: Tissue Homogenates Application: WB Targeting matriptase in breast cancer abrogates tumour progression via impairment of stromal-epithelial growth factor signalling. Authors: Zoratti G, Tanabe L, Varela F, Murray A, Bergum C, Colombo E, Lang J, Molinolo A, Leduc R, Marsault E, Boerner J, List K Nat Commun, 2015;6(0):6776. Species: Human Sample Type: Cell Lysate Application: WB Reduced prostasin (CAP1/PRSS8) activity eliminates HAI-1 and HAI-2 deficiency-associated developmental defects by preventing matriptase activation. Authors: Szabo, Roman, Uzzun Sales, Katiuchi, Kosa, Peter, Shylo, Natalia, Godiksen, Sine, Hansen, Karina K, Friis, Stine, Gutkind, J Silvio, Vogel, Lotte K, Hummler, Edith, Camerer, Eric, Bugge, Thomas H PLoS Genet, 2012;8(8):e1002937. Species: Mouse Sample Type: Tissue Homogenates Application: WB Endogenous expression of matriptase in neural progenitor cells promotes cell migration and neuron differentiation. Authors: Fang JD, Chou HC, Tung HH J. Biol. Chem., 2011;286(7):5667-79. Species: Mouse Sample Type: Cell Lysates Application: WB c-Met-induced epithelial carcinogenesis is initiated by the serine protease matriptase. Authors: Szabo R, Rasmussen AL, Moyer AB Oncogene, 2011;30(17):2003-16. Species: Mouse Sample Type: Cell Lysates Application: WB Matriptase initiates activation of epidermal pro-kallikrein and disease onset in a mouse model of Netherton syndrome. Authors: Sales KU, Masedunskas A, Bey AL Nat. Genet., 2010;42(8):676-83. Species: Mouse Sample Type: Whole Tissue Application: IHC Loss of matriptase suppression underlies spint1 mutation-associated ichthyosis and postnatal lethality. Authors: Szabo R, Kosa P, List K, Bugge TH Am. J. Pathol., 2009;174(6):2015-22. Species: Mouse Sample Type: recombinant matriptase Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human Matriptase/ST14 Catalytic Domain Val615-Val855 Accession # Q9Y5Y6
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Human matriptase, encoded by the ST14 (suppression of tumorogenicity 14) gene, is also known as tumor associated differentially expressed gene 15 protein/TADG‑15), epithin, and membrane-type serine protease 1/MT-SP1 (1). Predicted to have a significant role in tumor biology, matriptase may be a novel target for anti-cancer therapy (2). However, expressed in most human epithelia, matriptase is also important in several physiological processes (1). For example, it activates prostasin to initiate a protease cascade that is essential for epidermal differentiation (3), and it converts a single-chain IGFBP-rp1 into the two-chain form (4). Matriptase is a type II transmembrane serine protease with a complex modular structure (1). The 855 amino acid (aa) sequence of human matriptase consists of a cytoplasmic tail (aa 1-55), a transmembrane domain (aa 56-76), and an extracellular portion (aa 77-855). The latter contains the following domains: SEA (aa 86-201), two CUBs (aa 214-334 and 340-447), four LDLRAs (aa 452-486, 487-523, 524-560, and 566-603), and a serine protease (aa 615-855). The physiological activation of the single-chain zymogen requires the cleavage at the SEA domain within the ER or Golgi, association with HAI-1, which facilitates the transport of the protease to the cell surface, and auto-cleavage at QAR-V(615)VGG (1). The activated matriptase is inhibited by HAI-1, and the resulting HAI-1 complex can be shed from the cell surface (1). R&D Systems rhST14 corresponds to the catalytic domain, and is inhibited effectively by rhHAI-1 and rhHAI-2A (R&D Systems, Catalog # 1048-PI and 1106-PI).
运输条件	Blue Ice
存放说明	4℃
参考文献	List, K. et al. (2006) Mol. Med. 12:1. Uhland, K. (2006) Cell. Mol. Life Sci. 63:2968. Netzel-Arnett, S. et al. (2006) J. Biol. Chem. 281:32941. Ahmed, S. et al. (2006) FEBS J. 273:615.

参考图片

Detection of Matriptase/ST14 Catalytic Domain in PC‑3 Human Cell Line by Flow Cytometry. PC‑3 human prostate cancer cell line was stained with Sheep Anti-Human Matriptase/ST14 Catalytic Domain Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3946, filled histogram) or control antibody (Catalog # 5‑001‑A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.