| 货号 | AF393-SP |
| 别名 | Beta-TG; connective tissue-activating peptide III; CTAP III; CTAP3CTAP-III; CTAPIII; CXC chemokine ligand 7; C-X-C motif chemokine 7; CXCL7b-TG1; LA-PF4; LDGFTC1; Leukocyte-derived growth factor; low-affinity platelet factor IV; Macrophage-derived growth factor; MDGFTC2; NAP-2; NAP-2-L1; neutrophil-activating peptide 2; neutrophil-activating peptide-2; PBPTHBGB; platelet basic protein; PPBP; pro-platelet basic protein (chemokine (C-X-C motif) ligand 7); SCAR10; SCYB7beta-thromboglobulin; small inducible cytokine B7; small inducible cytokine subfamily B, member 7; Small-inducible cytokine B7; TGB; TGB1B-TG1; THBGB1; thrombocidin 1; thrombocidin 2; thromboglobulin, beta-1 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Simple Western(10 µg/mL) |
| 目标/特异性 | Detects human CXCL7/NAP‑2 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse (rm) MIP-2 is observed, 10% cross-reactivity with recombinant human (rh) GRO alpha, rhIL-8, rhCINC-2 alpha, and rhGRO beta is observed, and 5% cross-reactivity with recombinant rat (rr) CINC-1, rrCINC-2 beta, rhGCP-2, rmKC, rhMIG, and rhGRO beta. is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Simple Western: 10 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5473 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human CXCL7/NAP-2 Ala59-Asp128 Accession # P02775 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Neutrophil Activating Peptide 2 (NAP-2), Connective Tissue Activating Protein III (CTAP‑III) and beta -thrombogulin ( beta ‑TG), are proteolytically processed carboxyl-terminal fragments of platelet basic protein (PBP) which is found in the alpha-granules of human platelets. NAP-2 is a member of the CXC chemokines. Similar to other ELR domain containing CXC chemokines such as IL-8 and the GRO proteins, NAP-2 has been shown to bind CXCR2 and to chemoattract and activate neutrophils. Although CTAP-III, beta -TG and PBP represent amino-terminal extended variants of NAP-2 and possess the same CXC chemokine domains, these proteins do not exhibit NAP-2 activity. It has been shown that the additional amino-terminal residues of CTAP-III masks the critical ELR receptor binding domain that is exposed on NAP-2 and may account for lack of NAP-2 activity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human CXCL7/NAP‑2 by Simple WesternTM. Simple Western lane view shows lysates of human blood platelets, loaded at 0.2 mg/mL. A specific band was detected for CXCL7/NAP‑2 at approximately 7 kDa (as indicated) using 10 µg/mL of Goat Anti-Human CXCL7/NAP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF393) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
