| 货号 | AF3927-SP |
| 别名 | annexin A11; Annexin-11; ANX1156-kD; ANXA11; CAP50; CAP-50 |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects endogenous human, mouse, and rat Annexin A11 in Western blots. In Western blots, this antibody shows no cross-reactivity with recombinant human Annexin A1, A2, A3, A4, A5, A6, A7, A8, A9, A10, or A13. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 311 (Human); 11744 (Mouse); 290527 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Annexin A11 Met1-Asp505 Accession # P50995 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Annexin A11 (ANXA11), also known as Annexin XI, 56K Autoantigen and Calcyclin-associated annexin 50 (CAP50), is a 54.4 kDa (predicted) member of the Annexin protein family. The Annexins are a family of Calcium-dependent phospholipid-binding proteins that are preferentially located on the cytosolic face of the plasma membrane. The Annexin’s have a molecular weight of approximately 35‑40 kDa and consist of a unique amino terminal domain followed by a homologous C-terminal core domain containing the calcium-dependent phospholipid-binding sites. The C‑terminal domain is comprised of four 60‑70 amino acid (aa) repeats, known as annexin repeats or an endonexin fold (Annexin A6 contains 8 annexin repeats). The four annexin repeats form a highly alpha -helical, tightly packed disc known as the annexin domain, which binds to phospholipids in the membrane in a calcium-dependent manner. Members of the annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth, and intracellular signal transduction pathways. Annexin A11 was identified as a 56 kDa antigen recognized by sera from patients with autoimmune diseases such as rheumatoid arthritis, systemic lupus erythematosus, or Sjogrens syndrome. Human Annexin A11 shares 93% identity with mouse and rat Annexin A11. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse/Rat Annexin A11 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, A20 mouse B cell lymphoma cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Annexin A11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3927) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Annexin A11 at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. |
