| 货号 | AF3925-SP |
| 别名 | HEBP1; NFE2L2; NF-E2-related factor 2; NFE2-related factor 2; NRF2 erythroid derived 2, like 2; nuclear factor (erythroid-derived 2)-like 2; nuclear factor erythroid 2-related factor 2; nuclear factor erythroid-derived 2-like 2 | 全称 | NF-E2-related Factor 2 |
| 反应种属 | Human |
| 应用 | Western Blot(0.2 µg/mL) |
| 目标/特异性 | Detects human Nrf2 in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross-reactivity with recombinant mouse Nrf2 is observed. |
| 使用方法 | Western Blot: 0.2 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4780 (Human); 18024 (Mouse); 83619 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Nrf2 Met17-Asn605 Accession # Q16236 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | NF-E2 related factor 2 (Nrf2) is a member of the cap ‘n’ collar family of basic leucine zipper transcription factors. By binding antioxidant response elements (AREs), Nrf2 activates the transcription of genes encoding antioxidant and phase II drug-metabolizing enzymes needed to protect cells against oxidative damage. Transcriptional targets regulated by Nrf2 include genes encoding glutathione S-transferases, superoxide dismutase-1, and heme oxygenase-1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Nrf2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 10 µM MG 132 (Catalog # 1748) for 4 hours. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human Nrf2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3925) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Nrf2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
