| 货号 | AF3900-SP |
| 别名 | Protein Wnt-13; protein Wnt-2b; wingless-type MMTV integration site family, member 2B; Wnt-13; WNT13XWNT2, Xenopus, homolog of; XWNT2; XWNT2wingless-type MMTV integration site family, member 13 | 全称 | Wingless-type MMTV Integration Site Family Member 2b |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects recombinant human and recombinant mouse Wnt-2b in direct ELISAs and Western blots. In Western blots, approximately 20% cross-reactivity with recombinant human (rh) Wnt-2 is observed and less than 5% cross-reactivity with rhWnt-7a, -9a, recombinant mouse Wnt-1, -3a, -4, -5a, -5b, -8a, -8b, -9b, -10a, -10b, -11, and -16 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7482 (Human); 22414 (Mouse); 116466 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse Wnt‑2b Leu64-Glu104 and Arg251-Ala312 Accession # O70283 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Mouse Wnt-2b (also Wnt-13) is a 389 amino acid, 42kDa member of the large and highly conserved Wnt family of secreted, cysteine-rich glycoprotein signaling molecules. Wnt proteins are associated with developmental and carcinogenic processes. Wnt-2b may be necessary to maintain stem cells in an undifferentiated state. As a type II transmembrane protein with a long C-terminal extracellular and a short N-terminal cytoplasmic domain, Wnt-2b is presumably cleaved within its extracellular region to release the secreted form. In the region from aa 64‑104, mouse Wnt-2b is 100% identical to the human and rat protein, and over the 251‑312 aa region also used as immunogen, mouse Wnt-2b is 97% and 100% identical to the corresponding human and rat sequences, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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