| 货号 | AF3880-SP |
| 别名 | fatty acid binding protein 6, ileal; Fatty acid-binding protein 6; Gastrotropin; I-15P; I-15PIntestinal 15 kDa protein; IBABP; I-BABPIntestinal bile acid-binding protein; I-BALB; I-BAP; ILBP; ILBP3; ILBPGT; ileal bile acid binding protein; ILLBP; ILLBPIleal lipid-binding protein; illeal lipid-binding protein | 全称 | Fatty Acid-Binding Protein 6 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human FABP6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) FABP1, rhFABP2, rhFABP3, rhFABP4, rhFABP5, rhFABP7, and recombinant mouse FABP9 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2172 (Human); 16204 (Mouse); 25440 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human FABP6 Ala2-Ala128 Accession # P51161 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Fatty acid binding protein-6 (FABP6), also known as ileal bile acid binding protein (I-BABP) and gastrotropin, is a 15 kDa cytoplasmic protein that belongs to the FABP family. It is expressed in ileal epithelium and multiple other tissues. Alternate transcription promoters generate two transcript variants, encoding a 128 aa and a 177 aa residue protein. Human FABP6 isoform 2 contains 128 amino acid residues and is believed to be acetylated on Ala2. It binds both fatty acids and bile acids and has roles in fatty acid transport and metabolism. The amino acid sequence of human FABP6 is 80%, 78%, and 75% aa identical to that of mouse, canine, and porcine FABP6, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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FABP6 in T84 Human Cell Line. FABP6 was detected in immersion fixed T84 human colon carcinoma cell line using Goat Anti-Human FABP6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3880) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
