Human PDGF R beta Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human PDGF R beta Affinity Purified Polyclonal Ab (25 UG)

货号： AF385-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF385-SP
别名	beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; CD140b antigen; CD140b; EC 2.7.10; EC 2.7.10.1; JTK12; PDGFR; PDGFR1; PDGFRB; PDGF-R-beta; platelet-derived growth factor receptor, beta polypeptide
全称	Platelet-derived Growth Factor Receptor beta
反应种属	Human
应用	Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human PDGF R beta in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse PDGF R beta is observed, and less than 1% cross-reactivity with recombinant human PDGF sR alpha is observed.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize PDGF R beta inhibition of PDGF-BB-dependent proliferation in the NR6R‑3T3 mouse fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 10-40 µg/mL in the presence of 2 μg/mL Recombinant Human PDGF R beta Fc Chimera and 4 ng/mL Recombinant Human PDGF-BB.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5159 (Human); 18596 (Mouse); 24629 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Platelet-derived growth factor primes cancer-associated fibroblasts for apoptosis. Authors: Rizvi S, Mertens J, Bronk S, Hirsova P, Dai H, Roberts L, Kaufmann S, Gores G J Biol Chem, 2014;289(33):22835-49. Species: Human Sample Type: Cell Lysates Application: WB PDGFRbeta expression and function in fibroblasts derived from pluripotent cells is linked to DNA demethylation. J. Cell. Sci., 2012;125(0):2276-87. Species: Human Sample Type: Whole Cells Application: Neut Regulation of platelet-derived growth factor receptor function by integrin-associated cell surface transglutaminase. Authors: Zemskov EA, Loukinova E, Mikhailenko I, Coleman RA, Strickland DK, Belkin AM J. Biol. Chem., 2009;284(24):16693-703. Species: Human Sample Type: Cell Lysates Application: WB Inhibition of anastomotic intimal hyperplasia using a chimeric decoy strategy against NFkappaB and E2F in a rabbit model. Authors: Miyake T, Aoki M, Morishita R Cardiovasc. Res., 2008;79(4):706-14. Species: Rabbit Sample Type: Tissue Homogenates Application: WB Pancreatic stellate cells: partners in crime with pancreatic cancer cells. Authors: Vonlaufen A, Joshi S, Qu C, Phillips PA, Xu Z, Parker NR, Toi CS, Pirola RC, Wilson JS, Goldstein D, Apte MV Cancer Res., 2008;68(7):2085-93. Species: Human Sample Type: Whole Cells Application: ICC PDGF-C and -D induced proliferation/migration of human RPE is abolished by inflammatory cytokines. Authors: Li R, Maminishkis A, Wang FE, Miller SS Invest. Ophthalmol. Vis. Sci., 2007;48(12):5722-32. Species: Human Sample Type: Whole Cells Application: Neut C-KIT, by interacting with the membrane-bound ligand, recruits endothelial progenitor cells to inflamed endothelium. Authors: Dentelli P, Rosso A, Balsamo A, Colmenares Benedetto S, Zeoli A, Pegoraro M, Camussi G, Pegoraro L, Brizzi MF Blood, 2007;109(10):4264-71. Species: Human Sample Type: Whole Cells Application: Neut Vascular endothelial growth factor can signal through platelet-derived growth factor receptors. Authors: Ball SG, Shuttleworth CA, Kielty CM J. Cell Biol., 2007;177(3):489-500. Species: Human Sample Type: Whole Cells Application: Neut Heterodimerization of FGF-receptor 1 and PDGF-receptor-alpha: a novel mechanism underlying the inhibitory effect of PDGF-BB on FGF-2 in human cells. Authors: Faraone D, Aguzzi MS, Ragone G, Russo K, Capogrossi MC, Facchiano A Blood, 2006;107(5):1896-902. Species: Human Sample Type: Whole Cells Application: Neut Expression of growth factors and growth factor receptor in non-healing and healing ischaemic ulceration. Authors: Murphy MO, Ghosh J, Fulford P, Khwaja N, Halka AT, Carter A, Turner NJ, Walker MG Eur J Vasc Endovasc Surg, 2006;31(5):516-22. Species: Human Sample Type: Whole Tissue Application: IHC Glucose-induced phosphatidylinositol 3-kinase and mitogen-activated protein kinase-dependent upregulation of the platelet-derived growth factor-beta receptor potentiates vascular smooth muscle cell chemotaxis. Authors: Campbell M, Allen WE, Silversides JA, Trimble ER Diabetes, 2003;52(2):519-26. Species: Human Sample Type: Whole Cells Application: ICC Decorin inhibition of PDGF-stimulated vascular smooth muscle cell function: potential mechanism for inhibition of intimal hyperplasia after balloon angioplasty. Authors: Nili N, Cheema AN, Giordano FJ, Barolet AW, Babaei S, Hickey R, Eskandarian MR, Smeets M, Butany J, Pasterkamp G, Strauss BH Am. J. Pathol., 2003;163(3):869-78. Species: Rabbit Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human PDGF R beta Leu33-Phe530 (Glu241Asp) Accession # P09619
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF‑BB, and PDGF‑AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. Both PDGF R alpha and PDGF R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF, and Flt-3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and hetero-dimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Recombinant soluble PDGF R beta binds PDGF with high affinity and is potent PDGF antagonist.
运输条件	Blue Ice
存放说明	4℃
参考文献	Heldin, C.H. and L. Claesson-Welsh (1994) in Guidebook to Cytokines and Their Receptors, Nicola, N.A. ed. Oxford University Press, New York, p. 202.

参考图片

PDGF R beta in Human Breast Cancer Tissue.

PDGF R beta was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Goat Anti-Human PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF385) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

PDGF R beta Inhibition of PDGF-BB-dependent Cell Proliferation and Neutralization by Human PDGF R beta Antibody.

Recombinant Human PDGF R beta Fc Chimera (Catalog # 385-PR) inhibits Recombinant Human PDGF-BB (Catalog # 220‑BB) induced proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human PDGF-BB (4 ng/mL) activity elicited by Recombinant Human PDGF R beta Fc Chimera (2 μg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF385). The ND₅₀ is typically
10‑40 µg/mL.