| 货号 | AF3855-SP |
| 别名 | Cf2r; CF2RHTR; coagulation factor II (thrombin) receptor; Coagulation factor II receptor; F2R; HTR; PAR1; PAR1PAR-1protease-activated receptor 1; Protease-Activated Receptor 1; Thrombin receptor; ThrR; TRGPC; TRproteinase-activated receptor 1 | 全称 | Protease-Activated Receptor 1 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/106cells) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human PAR1 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/106cells Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2149 (Human); 14062 (Mouse); 25439 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Oncogenic epidermal growth factor receptor up-regulates multiple elements of the tissue factor signaling pathway in human glioma cells. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human PAR1 Arg27-Thr102, Ser375-Thr425 Accession # P25116 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Human Proteinase-Activated Receptor 1 (hPAR1), also known as thrombin receptor, is a 65-70 kDa, 399 amino acid long member of the seven-transmembrane superfamily of cell-surface G protein-coupled receptors. PAR1 is activated by thrombin cleavage of its N-terminal propeptide in the extracellular domain. Human PAR1 is widely expressed in many cell types including endothelial cells, and it has been implicated in a variety of inflammatory responses. Over the regions used as immunogen, human and mouse PAR1 proteins are 58% identical in the region spanning the propeptide and extracellular domains, and 84% identical in the cytoplasmic tail. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of PAR1 in HT‑29 Human Cell Line by Flow Cytometry. HT‑29 human colon adenocarcinoma cell line was stained with Human PAR1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3855, filled histogram) or isotype control antibody (Catalog # AB‑108‑C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). | |
PAR1 in HT‑29 Human Cell Line. PAR1 was detected in immersion fixed HT‑29 human colon adenocarcinoma cell line using Goat Anti-Human PAR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3855) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
