| 货号 | AF3854-SP |
| 别名 | N-WASPneural WiskNWASP; TRS4; WASL; WiskDKFZp779G0847; WiskMGC48327 | 全称 | Neural Wiskott-Aldrich Syndrome Protein |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human, mouse, and rat N-WASP in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8976 (Human); 73178 (Mouse); 296936 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human N-WASP Leu21-Asn270 Accession # O00401 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | N-WASP is a ubiquitously expressed member of the WASP family ofproteins. The 65 kDa, 505 amino acid, N-WASP seems to play a centralrole as a node interconnecting various actin networks. Over the regionused as immunogen, human N-WASP is greater than 98% identical to thecorresponding mouse and canine protein sequences. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human, Mouse, and Rat N-WASP by Western Blot. Western blot shows lysates of DU145 human prostate carcinoma cell line, Neuro‑2A mouse neuroblastoma cell line, and PC‑12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat N-WASP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3854) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for N-WASP at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
N‑WASP in Rat Brain Tissue. N‑WASP was detected in perfusion fixed frozen sections of rat brain (cortex) tissue using Goat Anti-Human/Mouse/Rat N‑WASP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3854) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to neuronal cell cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. |
