Human/Mouse Desmin Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human/Mouse Desmin Affinity Purified Polyclonal Ab (25 UG)

货号： AF3844-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF3844-SP
别名	CMD1I; CMD1IFLJ41013; CSM1; CSM2; DES; desmin; FLJ12025; FLJ39719; FLJ41793; intermediate filament protein
反应种属	Human/Mouse
应用	Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human and mouse Desmin.
使用方法	Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1674 (Human); 13346 (Mouse); 64362 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Ang-2/VEGF bispecific antibody reprograms macrophages and resident microglia to anti-tumor phenotype and prolongs glioblastoma survival Authors: J Kloepper, L Riedemann, Z Amoozgar, G Seano, K Susek, V Yu, N Dalvie, RL Amelung, M Datta, JW Song, V Askoxylaki, JW Taylor, C Lu-Emerson, A Batista, ND Kirkpatric, K Jung, M Snuderl, A Muzikansky, KG Stubenrauc, O Krieter, H Wakimoto, L Xu, LL Munn Proc Natl Acad Sci USA, 2016;113(16):4476-81. Species: Mouse Sample Type: Whole Tissue Application: IHC - Paraffin embedded Dual inhibition of Ang-2 and VEGF receptors normalizes tumor vasculature and prolongs survival in glioblastoma by altering macrophages Authors: TE Peterson, ND Kirkpatric, Y Huang, CT Farrar, KA Marijt, J Kloepper, M Datta, Z Amoozgar, G Seano, K Jung, WS Kamoun, T Vardam, M Snuderl, J Goveia, S Chatterjee, A Batista, A Muzikansky, CC Leow, L Xu, TT Batchelor, DG Duda, D Fukumura, RK Jain Proc Natl Acad Sci USA, 2016;113(16):4470-5. Species: Mouse Sample Type: Whole Tissue Application: IHC - Paraffin embedded Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling. Authors: Riordan D, Varma S, West R, Brown P PLoS ONE, 2015;10(7):e0128975. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded The novel desmin mutant p.A120D impairs filament formation, prevents intercalated disk localization, and causes sudden cardiac death. Authors: Brodehl A, Dieding M, Klauke B, Dec E, Madaan S, Huang T, Gargus J, Fatima A, Saric T, Cakar H, Walhorn V, Tonsing K, Skrzipczyk T, Cebulla R, Gerdes D, Schulz U, Gummert J, Svendsen J, Olesen M, Anselmetti D, Christensen A, Kimonis V, Milting H Circ Cardiovasc Genet, 2013;6(6):615-23. Species: Human Sample Type: whole cells Application: IHC Fresh Dual color photoactivation localization microscopy of cardiomyopathy-associated desmin mutants. Authors: Brodehl A, Hedde PN, Dieding M, Fatima A, Walhorn V, Gayda S, Ã…Â ariÃ„â€¡ T, Klauke B, Gummert J, Anselmetti D, Heilemann M, Nienhaus GU, Milting H J. Biol. Chem., 2012;287(19):16047-57. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human Desmin Val261-Leu470 Accession # P17661
生物活性	Human, Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Desmin is a 53 kDa, muscle-specific molecule that belongs to the intermediate filament superfamily of cytoskeletal proteins. Cytoplasmic Desmin homopolymers form stable filamentous networks that stabilize lateral alignments of myofibrils. Human Desmin is 470 amino acid (aa) peptide. It contains an N-terminal head segment (aa 1-84), multiple coiled regions (aa 109-412), and a tail region (aa 413-470). Single amino acid substitutions and three-amino acid deletions are associated with cardio‑skeletal myopathies. Most mutations affect filament assembly. Desmin is a highly conserved protein. Over the region used as immunogen, human Desmin is 99% identical to the corresponding mouse, canine, and porcine protein sequences.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cardiac Stem Cell Markers ICC / IHC Images: Desmin Intermediate Filaments Mesenchymal Cells and EMT Myogenesis Markers

参考图片

Desmin in Human Heart. Desmin was detected in immersion fixed paraffin-embedded sections of human heart using 15 µg/mL Human Desmin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3844) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the sarcoplasm of cardiomyocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Desmin in Mouse Skeletal Muscle. Desmin was detected in immersion fixed frozen sections of mouse skeletal muscle using Human Desmin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3844) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to z-lines. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.