| 货号 | AF3839-SP |
| 别名 | methyl-CpG binding domain protein 3; methyl-CpG-binding domain protein 3; Methyl-CpG-binding protein MBD3 | 全称 | Methyl-CpG Binding Domain Protein 3 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects human MBD3 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 53615 (Human); 17192 (Mouse); 314628 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human MBD3 isoform 2 Met1-Val259 Accession # AAH43619 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | MBD3 (methyl-CpG-binding domain protein # 3) is a 38‑40 kDa member of the MBD family of transcriptional repressors. Through its MBD, and as part of a large complex of regulatory proteins, it binds methylated DNA, promoting gene silencing during S phase. Although it can homodimerize, it is only active when heterodimerized with MBD2. Human MBD3 is 291 amino acids (aa) in length, and contains an N-terminal methyl binding domain (aa’s 1‑72) and a C-terminal coiled-coil region (aa’s 216‑245) that may be involved in protein-protein interaction. Human MBD3 shares 95% aa identity with mouse, rat, and canine MBD3, with differences essentially limited to the C‑terminal 25 amino acids. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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