Human Integrin beta 5 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human Integrin beta 5 Affinity Purified Polyclonal Ab (25 UG)

货号： AF3824-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF3824-SP
别名	FLJ26658; integrin beta-5; integrin, beta 5; ITGB5
反应种属	Human
应用	Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects human Integrin beta 5 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse (rm) Integrin beta 5 is observed, and less than 5% cross-reactivity with recombinant human (rh) Integrin beta 1, rhIntegrin beta 3, rhIntegrin beta 4, rhIntegrin beta 6 and rmIntegrin beta 7 is observed.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3693 (Human); 16419 (Mouse); 257645 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Development of a method for the purification and culture of rodent astrocytes. Authors: Foo LC, Allen NJ, Bushong EA, Ventura PB, Chung WS, Zhou L, Cahoy JD, Daneman R, Zong H, Ellisman MH, Barres BA Neuron, 2011;71(5):799-811. Species: Mouse Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	Chinese hamster ovary cell line CHO cell-derived recombinant human Integrin beta 5 Gly24-Asn719 Accession # P18084
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Integrin beta 5 is a 100‑110 kDa type I transmembrane glycoprotein that forms a non-disulfide-linked heterodimer with Integrin alpha _V. Integrin alpha _V beta 5 is a receptor for vitronectin that is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, and select endothelium. The 696 amino acid residue Integrin beta 5 extracellular region contains a vWF-A domain and a cysteine-rich tandem repeat region. Human Integrin beta 5 shares 91% and 89% amino acid sequence homology with mouse and canine Integrin beta 5, respectively.
运输条件	Blue Ice
存放说明	4℃
参考文献	Actin Stress Fibers and Focal Adhesions During EMT Adhesion Molecules Endothelial Cell Markers and Adhesion Molecules in Angiogenesis HGF/SF Activation of Integrins Integrin Ligands at a Glance (J. Cell. Sci.) Integrin Subunit Interactions Integrins and Associated Molecules

参考图片

Integrin beta 5 in MG‑63 Human Cell Line. Integrin beta 5 was detected in immersion fixed MG‑63 human osteosarcoma cell line using Sheep Anti-Human Integrin beta 5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3824) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.