货号 | AF3804-SP |
别名 | cell cycle regulated protein kinase; EC 2.7.11; EC 2.7.11.21; PLK; PLK-1; polo (Drosophia)-like kinase; polo like kinase; polo-like kinase 1polo-like kinase (Drosophila); Serine/threonine-protein kinase 13; serine/threonine-protein kinase PLK1; STPK13 | 全称 | Polo-like Kinase 1 |
反应种属 | Human |
应用 | Western Blot(1 µg/mL) |
目标/特异性 | Detects human PLK1 in Western blots. |
使用方法 | Western Blot: 1 µg/mL |
来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 5347 (Human); 18817 (Mouse); 25515 (Rat) |
纯化方式 | Antigen Affinity-purified |
免疫原 | E. coli-derived recombinant human PLK1 Ala400-Ser603 Accession # P53350 |
生物活性 | Human |
标记 | Unconjugated |
溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
背景 | Polo-like kinase-1 (PLK1) is a serine/threonine kinase that localizes to the mitotic spindle, and is involved in G2 and M phase cell cycle checkpoints. The expression of PLK1 is elevated in many different cancer types. Depletion of PLK1 in mammalian cells results in an inhibition of cell proliferation, decreased viability, and arrest in G2/M phase. PLK1 contains a polo-box domain that binds to phospho-serine and phospho-threonine residues. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of Human PLK1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Raji human Burkitts lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human PLK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3804) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PLK1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |