Human CD4 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human CD4 Affinity Purified Polyclonal Ab (25 UG)

货号： AF-379-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF-379-SP
别名	CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4
反应种属	Human
应用	Western Blot,Immunohistochemistry,Immunocytochemistry
目标/特异性	Detects human CD4 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse CD4 and recombinant rat CD4 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 493775 (Feline)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Tumor-specific cytotoxic T lymphocyte activity determines colorectal cancer patient prognosis. Authors: Reissfelder C, Stamova S, Gossmann C, Braun M, Bonertz A, Walliczek U, Grimm M, Rahbari N, Koch M, Saadati M, Benner A, Buchler M, Jager D, Halama N, Khazaie K, Weitz J, Beckhove P J Clin Invest, 2015;125(2):739-51. Species: Human Sample Type: Whole Tissue Application: IHC Frozen HIV-1 gp120 activates the STAT3/interleukin-6 axis in primary human monocyte-derived dendritic cells. Authors: Del Corno M, Donninelli G, Varano B, Da Sacco L, Masotti A, Gessani S J Virol, 2014;88(19):11045-55. Species: Human Sample Type: Whole Cells Application: Neut Slow turnover of HIV-1 receptors on quiescent CD4+ T cells causes prolonged surface retention of gp120 immune complexes in vivo. Authors: Suzuki, Yasuhiro, Gatanaga, Hiroyuki, Tachikawa, Natsuo, Oka, Shinichi PLoS ONE, 2014;9(2):e86479. Species: Human Sample Type: Whole Cells Application: ICC Patients with cystic fibrosis have inducible IL-17+IL-22+ memory cells in lung draining lymph nodes. Authors: Chan Y, Chen K, Duncan S, Lathrop K, Latoche J, Logar A, Pociask D, Wahlberg B, Ray P, Ray A, Pilewski J, Kolls J J Allergy Clin Immunol, 2013;131(4):1117-29, 1129. Species: Human Sample Type: Whole Tissue Application: IHC OCT-embedded A role for microRNA-155 modulation in the anti-HIV-1 effects of Toll-like receptor 3 stimulation in macrophages. Authors: Swaminathan G, Rossi F, Sierra L, Gupta A, Navas-Martin S, Martin-Garcia J PLoS Pathog, 2012;8(9):e1002937. Species: Human Sample Type: Whole Cells Application: ICC T cell infiltrates in the muscles of patients with dermatomyositis and polymyositis are dominated by CD28null T cells. Authors: Fasth AE, Dastmalchi M, Rahbar A, Salomonsson S, Pandya JM, Lindroos E, Nennesmo I, Malmberg KJ, Soderberg-Naucler C, Trollmo C, Lundberg IE, Malmstrom V J. Immunol., 2009;183(7):4792-9. Species: Human Sample Type: Whole Tissue Application: IHC Restricted T cell receptor BV gene usage in the lungs and muscles of patients with idiopathic inflammatory myopathies. Authors: Englund P, Wahlstrom J, Fathi M, Rasmussen E, Grunewald J, Tornling G, Lundberg IE Arthritis Rheum., 2007;56(1):372-83. Species: Human Sample Type: Whole Tissue Application: IHC Roles for herpes simplex virus type 1 UL34 and US3 proteins in disrupting the nuclear lamina during herpes simplex virus type 1 egress. Authors: Bjerke SL, Roller RJ Virology, 2006;347(2):261-76. Species: Human Sample Type: Whole Cells Application: ICC An effective cancer vaccine modality: lentiviral modification of dendritic cells expressing multiple cancer-specific antigens. Authors: Wang B, He J, Liu C, Chang LJ Vaccine, 2006;24(17):3477-89. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD4 Lys26-Trp390 Accession # P01730
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	CD4 is an approximately 55 kDa type I membrane glycoprotein that is expressed predominantly on most thymocytes and a subset of mature T lymphocytes. In humans, CD4 is also expressed to a lesser extent on monocytes and macrophage related cells. Human CD4 cDNA encodes a 458 amino acid (aa) residue precursor protein with a 25 aa residue signal peptide, a 371 aa residue extracellular region containing four immunoglobulin homology domains, a 24 aa residue transmembrane domain and a 38 aa residue cytoplasmic domain. CD4 is a coreceptor required for T cell recognition of antigens that are presented by class II major histocompatibility complexes. CD4 has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.
运输条件	Blue Ice
存放说明	4℃
参考文献	Capon, D.I.et al. (1991) Annu. Rev. Immunol. 9:649.

参考图片

CD4 in Human T Cells.

CD4 was detected in immersion fixed human T cells using 2 µg/mL Goat Anti-Human CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑379‑NA) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

CD4 in Human PBMCs.

CD4 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-379-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.