Human p300 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human p300 Affinity Purified Polyclonal Ab (25 UG)

货号： AF3789-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF3789-SP
别名	E1A binding protein p300; E1A-associated protein p300; E1A-binding protein, 300kD; EC 2.3.1; EC 2.3.1.48; EP300; histone acetyltransferase p300; KAT3B; p300 HAT; p300; RSTS2
全称	E1A-binding Protein p300
反应种属	Human
应用	Western Blot(1 µg/mL) Simple Western(50 µg/mL) Chromatin Immunoprecipitation (ChIP)(5 µg/5 x 10⁶cells)
目标/特异性	Detects human p300 in Western blots.
使用方法	Western Blot: 1 µg/mL Simple Western: 50 µg/mL Chromatin Immunoprecipitation (ChIP): 5 µg/5 x 10⁶cells Immunocytochemistry: 1-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2033 (Human); 328572 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Metabolic Reprogramming, Autophagy, and Reactive Oxygen Species Are Necessary for Primordial Germ Cell Reprogramming into Pluripotency Authors: D Sainz de l, A Moratilla, V Aparicio, C Lorca, Y Alcaina, D Martín, MP De Miguel Oxid Med Cell Longev, 2017;2017(0):4745252. Species: Mouse Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human p300 His2283-His2414 Accession # Q09472
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	The E1A-binding protein p300 is a histone acetyltransferase that also acts to acetylate other proteins, such as p53. p300 acts as a transcriptional coactivator that can serve as an adaptor molecule to bridge to transcriptional regulators. In addition, p300 binds to PCNA and may participate in chromatin remodeling.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cell Cycle Cell Structure and Signaling Molecules Checkpoint EMT Induction Epigenetics and EMT Intracellular Signaling Molecules in Angiogenesis Notch Pathway p53 Pathway Positive Regulators of the Jak/STAT Pathway

参考图片

Detection of Human p300 by Western Blot. Western blot shows nuclear extracts of A431 human epithelial carcinoma cell line epidermoid carcinoma and HeLa human cervical epithelial carcinoma cell line cervical carcinoma cell lines. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human p300 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3789) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for p300 at approximately 300 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Detection of p300-regulated Genes by Chromatin Immunoprecipitation. Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. p300/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human p300 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3789) or control antibody (Catalog #
AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The fos promoter was detected by standard PCR.

Detection of Human p300 by Simple Western^TM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for p300 at approximately 308 kDa (as indicated) using 50 µg/mL of Goat Anti-Human p300 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3789) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
66-440 kDa separation system.