| 货号 | AF3788-SP |
| 别名 | EC 1.17.4.1; MGC102856; MGC42116; MTDPS8A; MTDPS8B; p53-inducible ribonucleotide reductase small subunit 2 homolog; p53-inducible ribonucleotide reductase small subunit 2-like protein; p53R2; P53R2DKFZp686M05248; PEOA5; ribonucleoside-diphosphate reductase subunit M2 B; ribonucleotide reductase M2 B (TP53 inducible); RRM2B; TP53-inducible ribonucleotide reductase M2 B |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human p53R2 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 50484 (Human); 382985 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human p53R2 Met1-Lys180 Accession # Q7LG56 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The p53-inducible ribonucleotide reductase (p53R2) shares similarity with the R2 subunit of ribonucleotide reductase. Both function to provide dNTPs for DNA synthesis. p53R2 is induced in response to genotoxic stress, whereas the R2 functions under homeostatic conditions. When the expression of p53R2 is decreased, cells exhibit decreased ribonucleotide reductase activity, DNA repair activity, and cellular survival in response to genotoxins. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human p53R2 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line and U2OS human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Human p53R2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3788) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for p53R2 at approximately 45 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1. |
