| 货号 | AF3785-SP |
| 别名 | BUB1 budding uninhibited by benzimidazoles 1 homolog; BUB1Amitotic checkpoint serine/threonine-protein kinase BUB1; BUB1L; budding uninhibited by benzimidazoles 1 (yeast homolog); budding uninhibited by benzimidazoles 1 homolog (yeast); EC 2.7.11.1; hBUB1mitotic spindle checkpoint kinase; putative serine/threonine-protein kinase | 全称 | BUB1 Budding Uninhibited By Benzimidazoles 1 Homolog |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human Bub1 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 699 (Human); 12235 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Bub1 Met1-Ser250 Accession # O43683 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The Bub1 (Budding uninhibited by benzimidazoles-1) protein is a kinase that acts in the mitotic spindle checkpoint. Bub1 delays chromosome segregation in response to genotoxic stress. Mutations or the absence of Bub1 can lead to aneuploidy and chromosomal instability. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Bub1 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line and nuclear extracts of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human Bub1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3785) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for Bub1 was detected at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
