| 货号 | AF3776-SP |
| 别名 | bK286B10; EC 1.14.99.3; heat shock protein, 32-kD; heme oxygenase (decycling) 1; HMOX1; HO; HO1; HO-1heme oxygenase 1; HSP32 | 全称 | Heme Oxygenase 1 |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(0.5 µg/mL) Simple Western(25 µg/mL) |
| 目标/特异性 | Detects human and mouse HO‑1/HMOX1/HSP32 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human HO-2 is observed. |
| 使用方法 | Western Blot: 0.5 µg/mL Simple Western: 25 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3162 (Human); 24451 (Mouse); 15368 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Anti-inflammatory effects of ethyl acetate fraction from Melilotus suaveolens Ledeb on LPS-stimulated RAW 264.7 cells. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human HO‑1/HMOX1/HSP32 Met1-Thr261 Accession # P09601 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Heme Oxygenase 1 (HO-1), also known as HMOX1 and Heat Shock Protein 32 (HSP32), is a 32 kDa microsomal enzyme required for the metabolism of heme to biliverdin. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 (HO-1/HMOX1) and a constitutive heme oxygenase-2 (HO-2/HMOX2). HO-1 expression is induced by heme and other non-heme compounds. Human HO-1 shares 82% amino acid sequence identity with mouse HO-1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse HO‑1/HMOX1/ HSP32 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line, DU145 human prostate carcinoma cell line, and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human/Mouse HO‑1/HMOX1/HSP32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3776) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human HO-1 and HO-2 (5 ng/lane) were included. A specific band for HO-1/HMOX1/HSP32 was detected at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. | |
Detection of Human and Mouse HO‑1/HMOX1/HSP32 by Simple WesternTM. Simple Western lane view shows lysates of A20 mouse B cell lymphoma cell line and A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for HO‑1/HMOX1/HSP32 at approximately 37 kDa (as indicated) using 25 µg/mL of Goat Anti-Human/Mouse HO‑1/HMOX1/HSP32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3776) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
