Human/Mouse/Rat IKK alpha Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human/Mouse/Rat IKK alpha Affinity Purified Polyclonal Ab (25 UG)

货号： AF3768-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF3768-SP
别名	CHUK; conserved helix-loop-helix ubiquitous kinaseIKBKA; EC 2.7.11; EC 2.7.11.10; I-kappa-B kinase 1; I-kappa-B kinase alpha; IkappaB kinase; IkBKA; IkBKalpha; IKK1; IKK1I-kappa-B kinase-alpha; IKK-A; IKKAIkB kinase alpha subunit; IKK-alphainhibitor of nuclear factor kappa-B kinase subunit alpha; NFKBIKA; NFKBIKAIKK-a kinase; Nuclear factor NFkappaB inhibitor kinase alpha; Nuclear factor NF-kappa-B inhibitor kinase alpha; TCF16; TCF-16; Transcription factor 16
全称	IkB Kinase alpha
反应种属	Human/Mouse/Rat
应用	Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects human, mouse, and rat IKK alpha. Does not cross-react with recombinant human (rh) IKK beta, rhIKK gamma, or rhIKK epsilon.
使用方法	Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1147 (Human); 12675 (Mouse); 309361 (Rat)
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human IKK alpha Gly375-Glu745 Accession # O15111
生物活性	Human, Mouse, Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	I kappa B kinase alpha (IKK alpha ) is also known as IKBKA, CHUK, and IKK1. The classical active IKK complex, composed of IKK alpha, IKK beta, and two forms of processed IKK gamma, phosphorylates and inactivates I kappa B, resulting in the release and nuclear translocation of active NF-kappa B. IKK alpha contains kinase, leucine zipper, and helix-loop-helix domains, and heterodimerizes with IKK beta. NF-kappa B-inducing kinase (NIK) phosphorylates and activates IKK alpha.
运输条件	Blue Ice
存放说明	4℃
参考文献	Chemokine Receptor Intracellular Signaling C-type Lectin Receptors IL-1 Family IL-17 Family Intracellular Kinases Intracellular Kinases in the Akt Pathway Natural Killer T (NKT) Cells NFkB Pathway NOD-like Receptors and the Inflammasome Notch Pathway PDGF Family RIG-I-like Receptors Stem Cell Transcription Factor Regulators TNF Superfamily Regulators Toll-Like Receptors Transcriptional Co-Activators

参考图片

Detection of Human/Mouse/Rat IKK alpha by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line, Rat‑2 rat embryonic fibroblast cell line, and U937 human histiocytic lymphoma cell lines. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat IKK alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3768) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IKK alpha at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

IKK alpha in HCT‑116 Human Cell Line. IKK alpha was detected in immersion fixed HCT‑116 human colorectal carcinoma cell line using Sheep Anti-Human/Mouse/Rat IKK alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3768) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.