| 货号 | AF3766-SP |
| 别名 | EC 2.7.10; fyn-related kinaseProtein-tyrosine kinase 5; Gtk; GTKEC 2.7.10.2; Nuclear tyrosine protein kinase RAK; PTK5 protein tyrosine kinase 5; PTK5; Rak; RAKPTK5; tyrosine-protein kinase FRK | 全称 | FYN-related Kinase |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(2 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human, mouse, and rat Frk in Western blots. |
| 使用方法 | Western Blot: 2 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2444 (Human); 14302 (Mouse); 79209 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Frk Met1-Glu107 Accession # P42685 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Fyn-related kinase (Frk) is a tyrosine kinase of the Src family. Also known as Rak, Gtk, and Iyk, Frk is predominantly expressed in epithelial tissues. Frk protein is 49% and 47% identical to Fyn and Csk, respectively. Src family kinases regulate an array of cellular processes, including growth factor signaling, cytoskeleton dynamics, and cell proliferation. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Frk by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, K562 human chronic myelogenous leukemia cell line, and NTera‑2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse/Rat Frk Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3766) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Frk at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
