| 货号 | AF3760-SP |
| 别名 | MIZ1; Miz-1; Myc-interacting zinc finger protein 1; mZ13; pHZ-67; ZBTB17; Zfp100; zinc finger and BTB domain containing 17; zinc finger and BTB domain-containing protein 17; zinc finger protein 151 (pHZ-67); Zinc finger protein 151; Zinc finger protein 60MIZ-1; ZNF151; ZNF151Myc-interacting Zn finger protein-1; ZNF60 | 全称 | Myc-interacting Zinc Finger Protein-1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects endogenous human Miz-1 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7709 (Human); 22642 (Mouse); 313666 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Target genes of the largest human SWI/SNF complex subunit control cell growth. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Miz-1 Met1-Ala188 Accession # Q13105 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Miz-1 (Myc-interacting zinc finger protein 1; also ZBTB17) is an 88 kDa, DNA-binding protein that belongs to the Kruppel C2H2-type zinc finger protein family. Human Miz-1 is 803 amino acids (aa) in length, and contains a POZ domain (aa 1 - 104), two Myc-interaction segments (aa 269 - 308 & 637 - 718), and 12 C2H2-type zinc finger motifs (aa 306 - 637); also, there is one alternate splice form that shows an eight aa substitution for Thr769. Miz-1 induces cell arrest at G1, an effect mediated by its activation of the gene coding for p15INK4b. This effect is blocked by Myc, which displaces transcriptional coactivators bound to Miz-1. Notably, although the downregulation of Miz-1 may contribute to Myc-induced cell transformation, the deactivation of Miz-1 is absolutely essential for Myc-induced apoptosis. Over aa 1‑188, human Miz-1 is 85% aa identical to mouse Miz-1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Miz‑1/ZBTB17 by Western Blot. Western blot shows lysates of Raji human Burkitts lymphoma cell line and CEM human T-lymphoblastoid cell line. PVDF membrane was probed with 1 µg/mL of Human Miz‑1/ZBTB17 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3760) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Miz-1/ZBTB17 at approximately 100 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1. |
