| 货号 | AF3759-SP |
| 别名 | PRKCB; PRKCB1; PRKCB2 | 全称 | Protein Kinase C beta 1 |
| 反应种属 | Human/Rat |
| 应用 | Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human and rat PKC beta 1 in direct ELISAs. In direct ELISAs, this antibody shows less than 1% cross‑reactivity with rhPKCδ. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5579 (Human); 18751 (Mouse); 25023 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human PKC beta 1 Lys607-Val671 Accession # P05771 |
| 生物活性 | Human, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Protein Kinase C (PKC) beta 1 is a member of the PKC family of serine/threonine protein kinases. It is 671 amino acids (aa) in length with a predicted molecular mass of 77 kDa. Human PKC beta 1 shares 98% aa sequence identity with the mouse and rat orthologs. A second PKC beta splice variant, PKC beta 2, contains an alternate C-terminal sequence. Conventional PKC isoforms, including PKC beta 1, require calcium and phosphatidylserine, diacylglycerol (DAG), or exogenous phorbol esters for activation. PKC beta 1 plays a role in B cell receptor signaling and histone demethylation. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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PKC beta 1 in Rat Cortical Stem Cells. PKC beta 1 was detected in immersion fixed rat cortical stem cells using Goat Anti-Human/Rat PKC beta 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3759) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green, upper panel; Catalog # NL003) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
