| 货号 | AF3757-SP |
| 别名 | CSDD1; CSDD1lin-28A; FLJ12457; lin-28 homolog (C. elegans); lin-28 homolog A (C. elegans); LIN28; LIN-28; Lin-28A; LIN28RNA-binding protein LIN-28; Tex17; ZCCHC1; ZCCHC1protein lin-28 homolog A; Zinc finger CCHC domain-containing protein 1; zinc finger, CCHC domain containing 1 | 全称 | RNA-binding Protein LIN-28 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human LIN-28A in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 79727 (Human); 83557 (Mouse); 298542 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Activation of pluripotency genes in human fibroblast cells by a novel mRNA based approach. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human LIN-28A Met1-Asn209 Accession # Q9H9Z2 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Human LIN-28 (Protein lin-28 homolog A; CSDD1, LIN28 and ZCCHC1) is a 30 kDa (209 amino acids) cytoplasmic RNA-binding protein with an N-terminal cold shock domain and two C-terminal CCHC zinc finger domains. It is expressed by various undifferentiated embryonic cell types and is also present in adult cardiac and skeletal muscle. Expression of LIN-28 has been shown to be regulated by micro-RNA. Human LIN-28 shares 98% and 97% amino acid sequence homology with rat and mouse LIN-28, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human LIN‑28A by Western Blot. Western blot shows lysates of JAR human choriocarcinoma cell line and NTera‑2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human LIN‑28A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3757) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for LIN‑28A at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
LIN‑28A in BG01V Human Stem Cells. LIN‑28A was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human LIN‑28A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3757) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
LIN‑28A in D3 Mouse Stem Cells. LIN‑28A was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Human LIN‑28A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3757) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
