| 货号 | AF3754-SP |
| 别名 | dishevelled 3 (homologous to Drosophila dsh); dishevelled, dsh homolog 3 (Drosophila); Dishevelled-3; DSH homolog 3; DVL3; KIAA0208dishevelled-3; segment polarity protein dishevelled homolog DVL-3 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Dishevelled-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human Dishevelled-1 and recombinant human Dishevelled-2 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1857 (Human); 13544 (Mouse); 303811 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Dishevelled-3 His545-Gly654 Accession # Q92997 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Members of the Dishevelled family are cytoplasmic phosphoproteins, and orthologs of the Drosophila dishevelled segment polarity protein. Dishevelleds are expressed in multiple tissues where they are well known for their participation in Wnt signal transduction. They are modular proteins that connect Wnt ligand engagement with Frizzled receptors, to proteins involved in the stabilization of beta-catenin in the canonical Wnt pathway. Upon Wnt binding, Dishevelled becomes associated with the beta-catenin complex and blocks GSK-3 beta kinase activity. This leads to beta-catenin accumulation, the activation of LEF/TCF transcription factors, and transcription of their target genes. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human and Mouse Dishevelled-3 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Dishevelled‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3754) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Dishevelled-3 at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group XXX. |
