Human FAP Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human FAP Affinity Purified Polyclonal Ab (25 UG)

货号： AF3715-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF3715-SP
别名	170 kDa melanoma membrane-bound gelatinase; DKFZp686G13158; DPPIV; EC 3.4.21.-; FAP; FAPA; Fibroblast activation protein alpha; fibroblast activation protein, alpha; Integral membrane serine protease; Seprase
反应种属	Human
应用	Western Blot(0.5 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunoprecipitation(25 µg/mL)
目标/特异性	Detects human Fibroblast Activation Protein alpha /FAP in direct ELISAs and Western blots.
使用方法	Western Blot: 0.5 µg/mL Immunohistochemistry: 5-15 µg/mL Immunoprecipitation: 25 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2191 (Human); 14089 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Fibroblast activation protein augments progression and metastasis of pancreatic ductal adenocarcinoma Authors: A Lo, CP Li, EL Buza, R Blomberg, P Govindaraj, D Avery, J Monslow, M Hsiao, E Puré JCI Insight, 2017;2(19):. Species: Human Sample Type: Whole Tissue Application: IHC-P A synthetic urinary probe-coated nanoparticles sensitive to fibroblast activation protein ? for solid tumor diagnosis Authors: X Feng, Q Wang, Y Liao, X Zhou, Y Wang, W Liu, G Zhang Int J Nanomedicine, 2017;12(0):5359-5372. Species: Human Sample Type: Cell Lysates Application: WB Adrenergic-mediated increases in INHBA drive CAF phenotype and collagens Authors: AS Nagaraja, RL Dood, G Armaiz-Pen, Y Kang, SY Wu, JK Allen, NB Jennings, LS Mangala, S Pradeep, Y Lyons, M Haemmerle, KM Gharpure, NC Sadaoui, C Rodriguez-, C Ivan, Y Wang, K Baggerly, P Ram, G Lopez-Bere, J Liu, SC Mok, L Cohen, SK Lutgendorf, SW Cole, AK Sood JCI Insight, 2017;2(16):. Species: Mouse Sample Type: Whole Tissue Application: IHC Fibroblast Activation Protein (FAP) Accelerates Collagen Degradation and Clearance from Lungs in Mice Authors: MH Fan, Q Zhu, HH Li, HJ Ra, S Majumdar, DL Gulick, JA Jerome, DH Madsen, M Christofid, DW Speicher, WW Bachovchin, C Feghali-Bo, E Puré J. Biol. Chem., 2016;291(15):8070-89. Species: Mouse Sample Type: Tissue Homogenates Application: IP
纯化方式	Antigen Affinity-purified
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Fibroblast Activation Protein alpha /FAP Leu26-Asp760 Accession # Q12884
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	FAP (also known as seprase) is a Type II transmembrane serine protease that is structurally related to dipeptidyl peptidase IV (1). FAP has substrate specificity similar to dipeptidyl peptidase IV, which is specific for N-terminal Xaa-Pro sequences, but FAP is also an endopeptidase able to degrade gelatin and Type I collagen (2). The enzymatically active form of FAP is a dimer (3). FAP has a restricted tissue distribution. It is not detectable in normal tissues or resting fibroblasts, but is highly expressed on reactive stromal fibroblasts in epithelial cancers (4), in granulation tissue during wound healing, and in bone and soft tissue sarcomas (5). Because of its expression patterns and enzymatic activities, FAP is believed to play roles in tumor invasion, tissue remodeling, and wound repair.
运输条件	Blue Ice
存放说明	4℃
参考文献	Scanlan, M.J. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5657. Park, J.E. et al. (1999) J. Biol. Chem. 274:36505. Pineiro-Sanchez, M.L. et al. (1997) J. Biol. Chem. 272:7595. Garin-Chesa, P. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7235. Rettig, W.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3110.

参考图片

Detection of Human Fibroblast Activation Protein alpha /FAP by Western Blot. Western blot shows lysates of WI‑38 human lung fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Fibroblast Activation Protein alpha /FAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3715) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Fibroblast Activation Protein alpha /FAP at approximately 97 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Fibroblast Activation Protein alpha /FAP in Human Squamous Cell Carcinoma. Fibroblast Activation Protein alpha /FAP was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Sheep Anti-Human Fibroblast Activation Protein alpha /FAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3715) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to connective tissue. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Fibroblast Activation Protein alpha /FAP in Human Basal Cell Carcinoma. Fibroblast Activation Protein alpha /FAP was detected in immersion fixed paraffin-embedded sections of human basal cell carcinoma using Sheep Anti-Human Fibroblast Activation Protein alpha /FAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3715) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.