| 货号 | AF3697-SP |
| 别名 | EC 3.1.3; EC 3.1.3.48; KIAA0283RPTPrho; protein tyrosine phosphatase, receptor type, T; PTPRT; receptor protein tyrosine phosphatase; Receptor-type tyrosine-protein phosphatase rho; receptor-type tyrosine-protein phosphatase T; RPTP-rho; R-PTP-T | 全称 | Protein Tyrosine Phosphatase Receptor-type T |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human PTPρ/PTPRT in Western blots. In Western blots, no cross-reactivity with recombinant human LAR, PTPRM, K, S, G or DEP1/CD148 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 11122 (Human); 19281 (Mouse); 362263 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human PTPρ/PTPRT Gln26-Lys725 Accession # O14522 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Protein Tyrosine Phosphatase, Receptor-type T (PTPRT), also known as RPTPrho, dephosphorylates tyrosine residues in proteins. PTPRT is present in many tissues, including brain, heart, lung, and colon. Genetic screening of colon cancers indicates that the PTPRT gene has a high incidence of missense mutations that reduce phosphatase activity in the encoded protein. Overexpression of intact PTPRT in cancer cell lines inhibits cell proliferation, suggesting that the phosphatase is a tumor suppressor. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human PTPρ/PTPRT by Western Blot. Western blot shows lysates of HT‑29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human PTPρ/PTPRT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3697) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PTPρ/PTPRT at approximately 195 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4. |
