| 货号 | AF3696-SP |
| 别名 | avian myelocytomatosis viral oncogene homolog; BHLHE39; bHLHe39MRTL; Class E basic helix-loop-helix protein 39; c-Myc; myc proto-oncogene protein; Myc; Myc2; MYCC; myc-related translation/localization regulatory factor; Niard; Nird; Proto-oncogene c-Myc; Transcription factor p64; v-myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog (avian) | 全称 | v-Myc Avian Myelocytomatosis Viral Oncogene Homolog (Avian) |
| 反应种属 | Human |
| 应用 | Western Blot,Simple Western,Immunohistochemistry,Chromatin Immunoprecipitation (ChIP),Immunocytochemistry |
| 目标/特异性 | Detects human c-Myc in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) L-Myc and rhN-Myc is observed. |
| 使用方法 | Western Blot: 0.5 µg/mL Simple Western: 20 µg/mL Immunohistochemistry: 5-15 µg/mL Chromatin Immunoprecipitation (ChIP): 5 µg/5 x 106cells Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4609 (Human); 17869 (Mouse); 24577 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Convergence of cMyc and beta-catenin on Tcf7l1 enables endoderm specification. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human c-Myc Arg66-Asp201 Accession # P01106 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Human c-Myc is a 439 amino acid transcription factor with a bHLH/LZ(basic Helix-Loop-Helix, Leucine Zipper) domain. c-Myc DNA-binding andtranscription function is achieved upon heterodimerization with itspartner Max. c-Myc is often over-expressed and mutated in hematopoietictumors. Mutations frequently result in truncations that remove thetransactivation region or in the bHLH/LZ domain required forassociation with Max and DNA. Over the region used as immunogen, humanc-Myc is 92% identical to the rat and mouse c-Myc proteins. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human c-Myc by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human c-Myc Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3696) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for c-Myc at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of c‑Myc-regulated Genes by Chromatin Immunoprecipitation. HeLa human cervical epithelial carcinoma cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. c‑Myc/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human c‑Myc Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3696) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The p21 promoter was detected by standard PCR. | |
c‑Myc in BG01V Human Stem Cells. c‑Myc was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human c‑Myc Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3696) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
c‑Myc in D3 Mouse Stem Cells. c‑Myc was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Human c‑Myc Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3696) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
Detection of Human c‑Myc by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for c‑Myc at approximately 78 kDa (as indicated) using 20 µg/mL of Goat Anti-Human c‑Myc Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3696) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
