| 货号 | AF3668-SP |
| 别名 | DJ-1; DJ1FLJ34360; EC 3.4; FLJ27376; FLJ92274; Oncogene DJ1; Parkinson disease (autosomal recessive, early onset) 7; Parkinson disease protein 7; protein DJ-1 | 全称 | Parkinson Disease 7 |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(0.5 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human and mouse Park7/DJ-1 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 11315 (Human); 57320 (Mouse); 117287 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse Park7/DJ-1 Ala2-Asp189 Accession # Q99LX0 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Park7, also known as DJ-1, is a cytoplasmic protein that belongs to theThiJ/Pfp1/DJ-1 superfamily of highly conserved proteins that functionas protein chaperones, catalases, proteases and kinases. Park7 iswidely expressed in the brain as well as in peripheral tissues. Itexists as a homodimer that can be localized in the cytoplasm, nucleusand mitochondria. Park7 is a redox-sensitive protein that has beenascribed various functions including that as a redox sensor andantioxidant protein. Mutations in Park7 are associated with a smallpercentage of hereditary early onset Parkinson’s disease. Human andmouse Park7 share 92% amino acid sequence identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human/Mouse Park7/DJ‑1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, U937 human histiocytic lymphoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse Park7/DJ‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3668) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Park7/DJ‑1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Park7/DJ‑1 in HeLa Human Cell Line. Park7/DJ‑1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse Park7/DJ‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3668) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
