Human CX3CL1/Fractalkine Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human CX3CL1/Fractalkine Affinity Purified Polyclonal Ab (25 UG)

货号： AF365-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF365-SP
别名	ABCD-3; C3Xkine; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; CXC3; CXC3C; FKN; Fractalkine; Neurotactin; neurotactin); NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine; NTTSmall-inducible cytokine D1; SCYD1C-X3-C motif chemokine 1; small inducible cytokine subfamily D (Cys-X3-Cys), member-1
反应种属	Human
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human CX3CL1/Fractalkine in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant mouse CX3CL1 and recombinant rat CX3CL1 is observed and less than 5% cross-reactivity with recombinant human (rh) MCP‑2 and rhMCP-4 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize CX3CL1/Fractalkine-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CX3CR1. The Neutralization Dose (ND₅₀) is typically 0.3-1.5 µg/mL in the presence of 100 ng/mL Recombinant Human CX3CL1/Fractalkine.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6376 (Human); 20312 (Mouse); 89808 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The Fractalkine-Receptor Axis Improves Human Colorectal Cancer Prognosis by Limiting Tumor Metastatic Dissemination. Authors: Erreni M, Siddiqui I, Marelli G, Grizzi F, Bianchi P, Morone D, Marchesi F, Celesti G, Pesce S, Doni A, Rumio C, Roncalli M, Laghi L, Mantovani A, Allavena P J Immunol, 2016;196(2):902-14. Species: Human Sample Type: Whole Tissue Application: IHC - Paraffin embedded Expression and regulation of chemokines in murine and human type 1 diabetes. Authors: Sarkar SA, Lee CE, Victorino F, Nguyen TT, Walters JA, Burrack A, Eberlein J, Hildemann SK, Homann D Diabetes, 2012;61(2):436-46. Species: Human Sample Type: Whole Cells Application: Flow Fractalkine is a novel human adipochemokine associated with type 2 diabetes. Authors: Shah R, Hinkle CC, Ferguson JF, Mehta NN, Li M, Qu L, Lu Y, Putt ME, Ahima RS, Reilly MP Diabetes, 2011;60(5):1512-8. Species: Human Sample Type: Whole Cells Application: Neut Thromboxane prostanoid receptor stimulation induces shedding of the transmembrane chemokine CX3CL1 yet enhances CX3CL1-dependent leukocyte adhesion. Authors: Tole S, Durkan AM, Huang YW, Liu GY, Leung A, Jones LL, Taylor JA, Robinson LA Am. J. Physiol., Cell Physiol., 2010;298(6):C1469-80. Species: Human Sample Type: Cell Lysates Application: WB An engineered CX3CR1 antagonist endowed with anti-inflammatory activity. Authors: Dorgham K, Ghadiri A, Hermand P, Rodero M, Poupel L, Iga M, Hartley O, Gorochov G, Combadiere C, Deterre P J. Leukoc. Biol., 2009;86(4):903-11. Species: Human Sample Type: phage library Application: ELISA Development Constitutive endocytosis of the chemokine CX3CL1 prevents its degradation by cell surface metalloproteases. Authors: Huang YW, Su P, Liu GY, Crow MR, Chaukos D, Yan H, Robinson LA J. Biol. Chem., 2009;284(43):29644-53. Species: Human Sample Type: Whole Cells Application: ICC CX3CR1 is expressed by prostate epithelial cells and androgens regulate the levels of CX3CL1/fractalkine in the bone marrow: potential role in prostate cancer bone tropism. Authors: Jamieson WL, Shimizu S, DAmbrosio JA, Meucci O, Fatatis A Cancer Res., 2008;68(6):1715-22. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Fractalkine and CX3CR1 mediate leukocyte capture by endothelium in response to Shiga toxin. Authors: Zanchi C, Zoja C, Morigi M, Valsecchi F, Liu XY, Rottoli D, Locatelli M, Buelli S, Pezzotta A, Mapelli P, Geelen J, Remuzzi G, Hawiger J J. Immunol., 2008;181(2):1460-9. Species: Human Sample Type: Cell Lysates Application: WB Fractalkine, a CX3C chemokine, as a mediator of ocular angiogenesis. Authors: You JJ, Yang CH, Huang JS, Chen MS, Yang CM Invest. Ophthalmol. Vis. Sci., 2007;48(11):5290-8. Species: Bovine Sample Type: Whole Cells Application: Neut Down-regulation of surface fractalkine by RNA interference in B16 melanoma reduced tumor growth in mice. Authors: Ren T, Chen Q, Tian Z, Wei H Biochem. Biophys. Res. Commun., 2007;364(4):978-84. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Immunohistochemical assessment of fractalkine, inflammatory cells, and human herpesvirus 7 in human salivary glands. Authors: Latchney LR, Fallon MA, Culp DJ, Gelbard HA, Dewhurst S J. Histochem. Cytochem., 2004;52(5):671-81. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Human cytomegalovirus-specific CD4+-T-cell cytokine response induces fractalkine in endothelial cells. Authors: Bolovan-Fritts CA, Trout RN, Spector SA J. Virol., 2004;78(23):13173-81. Species: Human Sample Type: Cell Lysates Application: WB Detection of fractalkine in human seminal plasma and its role in infertile patients. Authors: Zhang Q, Shimoya K, Ohta Y, Chin R, Tenma K, Isaka S, Nakamura H, Koyama M, Azuma C, Murata Y Hum. Reprod., 2002;17(6):1560-4. Species: Human Sample Type: seminal plasma Application: WB The transmembrane form of the CX3CL1 chemokine fractalkine is expressed predominantly by epithelial cells in vivo. Authors: Lucas AD, Chadwick N, Warren BF, Jewell DP, Gordon S, Powrie F, Greaves DR Am. J. Pathol., 2001;158(3):855-66. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human CX3CL1/Fractalkine Gln25-Gly100 Accession # Q6I9S9
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	CX3CL1, also named neurotactin, is a novel chemokine identified through bioinformatics. CX3CL1 has a unique C-X₃-C cysteine motif near the amino-terminus and is the first member of a fourth branch of the chemokine superfamily. Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. Human CX3CL1 cDNA encodes a 397 amino acid (aa) residue membrane protein with a 24 aa residue predicted signal peptide, a 76 aa residue chemokine domain, a 241 aa residue stalk region containing 17 degenerate mucin-like repeats, a 19 aa residue transmembrane segment and a 37 aa residue cytoplasmic domain. The extracellular domain of human CX3CL1 can be released, possibly by proteolysis at the dibasic cleavage site proximal to the membrane, to generate soluble CX3CL1. CX3CL1 mRNA has been detected in various tissues including the brain and heart. The expression of CX3CL1 was also reported to be up-regulated in endothelial cells and microglia by inflammatory signals. Membrane-bound CX3CL1 has been shown to promote adhesion of leukocytes. The soluble chemokine domain of human CX3CL1 was reported to be chemotactic for T cells and monocytes while the soluble chemokine domain of mouse CX3CL1 was reported to chemoattract neutrophils and T-lymphocytes but not monocytes. The gene for human CX3CL1 has been mapped to chromosome 16q.
运输条件	Blue Ice
存放说明	4℃
参考文献	Pan, Y. et al. (1997) Nature 387:611. Bazan, J.F. et al. (1997) Nature 385:640. Mackay, C.R. (1997) Current Biology 7:R384.

参考图片

Chemotaxis Induced by CX3CL1/Fractalkine and Neutralization by Human CX3CL1/Fractalkine Antibody. Recombinant Human CX3CL1/Fractalkine (Catalog # 365-FR) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CX3CL1/Fractalkine (100 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF365). The ND₅₀ is typically 0.3-1.5 µg/mL.

CX3CL1/Fractalkine in Human Breast Cancer Tissue. CX3CL1/Fractalkine was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Goat Anti-Human CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF365) overnight at 4 °C. Tissue was stained (red) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.