Human TLR9 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human TLR9 Affinity Purified Polyclonal Ab (25 UG)

货号： AF3658-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF3658-SP
别名	CD289 antigen; CD289; toll-like receptor 9
全称	Toll-like Receptor 9
反应种属	Human
应用	Flow Cytometry(2.5 µg/10⁶cells) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human TLR9 in direct ELISAs. In direct ELISAs, approximately 75% cross-reactivity with recombinant mouse TLR9 is observed, and less than 1% cross-reactivity with recombinant human (rh) TLR1, rhTLR2, rhTLR3, rhTLR4, rhTLR5, rhTLR7, rhTLR8, and rhTLR10 is observed.
使用方法	Flow Cytometry: 2.5 µg/10⁶cells Immunohistochemistry: 5-15 µg/mL
来源	Sterile PBS to a final concentration of 0.2 mg/mL.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	54106 (Human); 81897 (Mouse); 338457 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Fc?RIIIa Signaling Modulates Endosomal TLR Responses in Human CD4(+) T Cells Authors: AK Chauhan J. Immunol., 2017;0(0):. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human TLR9 Asn64-Glu189 Accession # Q9NR96
生物活性	Human
标记	Unconjugated
溶解方法	Sterile PBS to a final concentration of 0.2 mg/mL.
背景	TLRs make up a family of pattern recognition receptors that play important roles in the innate immune response. Broad classes of pathogens (e.g. viruses, bacteria, and fungi) constitutively express a set of mutation-resistant molecules called pathogen-associated molecular patterns (PAMPs). These microbial molecular markers may be composed of proteins, carbohydrates, lipids, nucleic acids and/or combinations thereof. Individual TLRs recognize distinct pathogen-associated PAMPs, initiating signaling cascades that promote the immune response. Structurally, TLRs are type I transmembrane receptors that possess varying numbers of extracellular N-terminal leucine-rich repeat (LRR) motifs, followed by a cysteine-rich region, a TM domain, and an intracellular Toll/IL-1 R (TIR) motif. The TIR motif is common to the larger IL-1 R/TLR superfamily.
运输条件	Blue Ice
存放说明	4℃
参考文献	B Cell Activation BIObrief: Pattern Recognition Receptors & the Innate Immune Response Human CD Marker Products IL-1 Family Innate Immunity and the Toll-like Receptor Family Interactive Pathway: Inflammasome Activation Pathways Interactive Pathway: Toll-Like receptor Signaling Pathways Mini-review: The Toll-like Receptor Family Neuroinflammation NFkB Pathway NOD-like Receptors and the Inflammasome Parturition & Surfactant Protein A Pattern Recognition Receptors Plasmacytoid Dendritic Cells TLR4: Contributing to Metabolic Syndrome in Multiple Tissues Toll-Like Receptors

参考图片

Detection of TLR9 in Human PBMC lymphocytes by Flow Cytometry. Human PBMC lymphocytes were stained with Sheep Anti-Human TLR9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3658) followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011) and Mouse Anti-Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P). Quadrant markers were set based on control antibody staining (Catalog # 5-001-A).

TLR9 in Human Tonsil. TLR9 was detected in immersion fixed paraffin-embedded sections of human tonsil using Sheep Anti-Human TLR9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3658) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to membranes of lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.