| 货号 | AF3646-SP |
| 别名 | bet; delta and Notch-like epidermal growth factor-related receptor; delta/notch-like EGF repeat containing; delta-notch-like EGF repeat-containing transmembrane; H_NH0150O02.1; UNQ26; WUGSC:H_NH0150O02.1 | 全称 | Delta/Notch-like EGF Repeat-containing Transmembrane |
| 反应种属 | Human |
| 应用 | Western Blot(0.2 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human DNER in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant mouse DNER is observed. |
| 使用方法 | Western Blot: 0.2 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 92737 (Human); 227325 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human DNER Arg29-His637 Accession # Q8NFT8 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | DNER, also known as brain EGF repeat containing transmembrane protein (BET), is a neuron-specific type I transmembrane notch ligand that is expressed on membranes of dendrites and cell bodies but not axons. It contains ten extracellular EGF-like domains and one follistatin-like domain (between the 9th and 10th EGF-like domains). DNER mediates neuron-glia interactions and is required for the functional and morphological maturation of the cerebellum. The extracellular domains of human and mouse DNER share 89% amino acid sequence homology. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Human DNER by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human DNER Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3646) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for DNER at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
