货号 | AF3637-SP |
别名 | FLJ32104; KOX17; RSG-A; Zfp191; zinc finger protein 488; ZSCAN3 | 全称 | Zinc Finger Protein 24 |
反应种属 | Human |
应用 | Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
目标/特异性 | Detects human ZNF24 in direct ELISAs and Western blots. |
使用方法 | Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL |
来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 7572 (Human); 331360 (Mouse) |
应用文献 | |
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Transcriptional repression of VEGF by ZNF24: mechanistic studies and vascular consequences in vivo. | |
纯化方式 | Antigen Affinity-purified |
免疫原 | E. coli-derived recombinant human ZNF24 Ser2-Val368 Accession # NP_008896 |
生物活性 | Human |
标记 | Unconjugated |
溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
背景 | Human ZNF24, also known as ZNF191, is a 42 kDa transcription regulator that belongs to the Kruppel-like zinc finger gene family. It contains an N-terminal SCAN box domain and four C-terminal tandem zinc finger motifs. ZNF24 is ubiquitously expressed. A second splice isoform that contains a 4 residue substitution for aa 191‑368 of full-length ZNF24, has been reported. Human and mouse ZNF24 share 95% amino acid sequence homology. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
ZNF24 in HepG2 Human Cell Line. ZNF24 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human ZNF24 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3637) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (left panel, red; Catalog # NL001) and counterstained with DAPI (right panel, blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |