| 货号 | AF3619-SP |
| 别名 | FKHL13; FKHL13forkhead transcription factor HFH-4; forkhead box J1; forkhead-like 13; Forkhead-related protein FKHL13; Hepatocyte nuclear factor 3 forkhead homolog 4; HFH-4; HFH4fork head homologue 4; HFH-4forkhead box protein J1; MGC35202 | 全称 | Forkhead Box J1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human FoxJ1 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2302 (Human); 15223 (Mouse); 116557 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Gefitinib, an EGFR Tyrosine Kinase inhibitor, Prevents Smoke-Mediated Ciliated Airway Epithelial Cell Loss and Promotes Their Recovery | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human FoxJ1 Lys306-Leu421 Accession # Q92949 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | FoxJ1 (Forkhead box protein J1; also HFH4) is a 55-65 kDa, class 1 member of the HNF-3/forkhead gene family of transcription factors. Its observed MW is higher than its predicted MW (45 kDa), suggesting anomalous migration on SDS-PAGE. FoxJ1 promotes the expression of cilia in respiratory, oviduct and choroid plexus epithelium, and maintains T cell tolerance to self-antigens. Human FoxJ1 is 421 amino acids (aa) in length, and contains one forkhead DNA binding domain (aas 120‑210). The presence of basic residues in the forkhead domain makes FoxJ1 a class 1 Fox protein. There is potential splice variant that contains a 25 aa substation for aa 262-287. Over aa 306-421, human FoxJ1 shares 88% aa with mouse FoxJ1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human FoxJ1 by Western Blot. Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human FoxJ1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3619) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for FoxJ1 at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
FoxJ1 in HEK293 Human Cell Line. FoxJ1 was detected in immersion fixed HEK293 human embryonic kidney cell line using Goat Anti-Human FoxJ1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3619) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
FoxJ1 in Early Proximal Lung Progenitors. FoxJ1 was detected in immersion fixed BGO1V human embryonic stem cells differentiated to early proximal lung progenitors using Goat Anti-Human FoxJ1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3619) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips. |
