| 货号 | AF3616-SP |
| 别名 | 1190004M21Rik; FLJ33226; PYGO2; pygopus 2; pygopus homolog 2 (Drosophila); pygopus homolog 2 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(3-15 µg/mL) |
| 目标/特异性 | Detects human Pygopus-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human Pygopus‑1 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 3-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 90780 (Human); 68911 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Pygopus-2 Met160-Glu336 Accession # Q9BRQ0 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Pygopus-2 is a 41 kDa nuclear protein that functions as atranscriptional co-activator in the Wnt signaling pathway. HumanPygopus-2 is synthesized as a 406 amino acid (aa) residue protein thathas the conserved N-terminal homology domain (NHD) and the C-terminalPHD (Plant homeodomain) zinc finger. The PHD zinc finger bindsLegless/Bcl9, which in turn binds beta -Catenin, which recruits TCF/LEF DNAbinding proteins. Formulation of the quaternary complex is required forthe activation of Wnt-responsive genes. Over the region used forimmunization, human Pygopus-2 shares 95% amino acid sequence identitywith the mouse protein. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Pygopus‑2 by Western Blot. Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Pygopus‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3616) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Pygopus‑2 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
Pygopus‑2 in Human Ovary and Human Ovarian Cancer Tissue. Pygopus‑2 was detected in immersion fixed paraffin-embedded sections of normal human ovary (left panel) and ovarian cancer tissue (right panel) using Goat Anti-Human Pygopus‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3616) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
