| 货号 | AF3605-SP |
| 别名 | ras-related protein R-Ras2; related RAS viral (r-ras) oncogene homolog 2; RRAS2; TC21Ras-like protein TC21; Teratocarcinoma oncogene | 全称 | Teratocarcinoma Oncogene TC21 |
| 反应种属 | Human/Rat |
| 应用 | Western Blot(2 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human and rat TC21/R-Ras2 in Western blots. Does not detect recombinant human R-Ras or M-Ras (R‑Ras3). |
| 使用方法 | Western Blot: 2 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 22800 (Human); 66922 (Mouse); 365355 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human TC21/R-Ras2 Met1-Phe204 Accession # P62070 |
| 生物活性 | Human, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Teratocarcinoma oncogene 21 (TC21), also known as R-Ras2, is a small GTP-binding protein of the Ras superfamily of GTPases. TC21 comprises an R-Ras subfamily with fellow members R-Ras and M-Ras (R-Ras3). Overexpression and activating mutations of TC21 have been reported in several tumor tissues and cell lines. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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TC21/R‑Ras2 in Human Placenta. TC21/R‑Ras2 was detected in immersion fixed paraffin-embedded sections of normal human placenta using Goat Anti-Human/Rat TC21/R‑Ras2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3605) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
Detection of Human/Rat TC21/R‑Ras2 by Western Blot. Western blot shows lysates of Rat-2 rat embryonic fibroblast cell line and CHP-100 human neuroblastoma cell line. PVDF membrane was probed with 2 µg/mL Goat Anti-Human/Rat TC21/R‑Ras2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3605) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human R-Ras, TC21/R-Ras2, and M-Ras/R-Ras3 (2 ng/lane) were included. A specific band for TC21/R-Ras2 was detected at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
