| 货号 | AF3598-SP |
| 别名 | FLJ20086; KIAA1584; MGC21637; MGC61687; SUHW4; Suppressor of hairy wing homolog 4; ZFP42; zinc finger protein 280D; Zinc finger protein 634; ZNF634suppressor of hairy wing homolog 4 (Drosophila) | 全称 | Zinc Finger Protein 42 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Rex‑1/ZFP42 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human YYI is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 132625 (Human); 22702 (Mouse); 290765 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Hyaluronan-CD44 interaction activates stem cell marker Nanog, Stat-3-mediated MDR1 gene expression, and ankyrin-regulated multidrug efflux in breast and ovarian tumor cells. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Rex‑1/ZFP42 Ser2-Lys150 Accession # NP_777560 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Rex-1, also named zinc finger protein 42 (Zfp-42), is a 32 kDa transcription factor belonging to the kruppel C2H2-type zinc-finger protein family. It is a putative stem cell marker and is highly expressed in embryonic and adult stem cells. Over the region used for immunization, human and mouse Rex-1 share 47% amino acid sequence identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Rex‑1/ZFP42 in BG01V Human Stem Cells. Rex‑1/ZFP42 was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Sheep Anti-Human Rex‑1/ZFP24 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3598) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
Rex‑1/ZFP42 in NTera‑2 Human Cell Line. Rex‑1/ZFP42 was detected in immersion fixed NTera‑2 human testicular embryonic carcinoma cell line using Sheep Anti-Human Rex‑1/ZFP42 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3598) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
