Human VEGF R2/KDR Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human VEGF R2/KDR Affinity Purified Polyclonal Ab (25 UG)

货号： AF357-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF357-SP
别名	CD309 antigen; CD309; EC 2.7.10; EC 2.7.10.1; Fetal liver kinase 1; fetal liver kinase-1; Flk-1; FLK1tyrosine kinase growth factor receptor; KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; vascular endothelial growth factor receptor 2; VEGFR; VEGFR2; VEGFR-2
全称	Vascular Endothelial Growth Factor Receptor 2
反应种属	Human
应用	Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human VEGF R2/KDR/Flk‑1 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse VEGF R2 is observed and less than 10% cross-reactivity with recombinant human (rh) VEGF R1 and rhVEGF R3 is observed.
使用方法	Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize VEGF R2/KDR/Flk‑1-mediated inhibition of proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND₅₀) is typically 0.05-0.25 µg/mL in the presence of 30 ng/mL Recombinant Human VEGF R2/KDR/Flk‑1 Fc Chimera and 5 ng/mL Recombinant Human VEGF₁₆₅.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3791 (Human); 16542 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Differential sensitivity of prostate tumor derived endothelial cells to sorafenib and sunitinib. Authors: Fiorio Pla, Alessand, Brossa, Alessia, Bernardini, Michela, Genova, Tullio, Grolez, Guillaum, Villers, Arnaud, Leroy, Xavier, Prevarskaya, Natalia, Gkika, Dimitra, Bussolati, Benedett BMC Cancer, 2014;14(0):939. Species: Human Sample Type: Cell Lysate Application: WB Differential expression of Vegfr-2 and its soluble form in preeclampsia. Authors: Munaut C, Lorquet S, Pequeux C, Coulon C, Le Goarant J, Chantraine F, Noel A, Goffin F, Tsatsaris V, Subtil D, Foidart JM PLoS ONE, 2012;7(3):e33475. Species: Human Sample Type: Plasma Application: WB The Down syndrome critical region gene 1 short variant promoters direct vascular bed-specific gene expression during inflammation in mice. Authors: Minami T, Yano K, Miura M, Kobayashi M, Suehiro J, Reid PC, Hamakubo T, Ryeom S, Aird WC, Kodama T J. Clin. Invest., 2009;119(8):2257-70. Species: Human Sample Type: Whole Cells Application: Neut Vascular endothelial growth factor drives autocrine epithelial cell proliferation and survival in chronic rhinosinusitis with nasal polyposis. Authors: Lee HS, Myers A, Kim J Am. J. Respir. Crit. Care Med., 2009;180(11):1056-67. Species: Human Sample Type: Whole Tissue Application: IHC Placenta growth factor in diabetic wound healing: altered expression and therapeutic potential. Authors: Cianfarani F, Zambruno G, Brogelli L, Sera F, Lacal PM, Pesce M, Capogrossi MC, Failla CM, Napolitano M, Odorisio T Am. J. Pathol., 2006;169(4):1167-82. Species: Human Sample Type: Cell Lysates Application: WB Enhancement of angiogenic effectors through hypoxia-inducible factor in preterm primate lung in vivo. Authors: Asikainen TM, Waleh NS, Schneider BK, Clyman RI, White CW Am. J. Physiol. Lung Cell Mol. Physiol., 2006;291(4):L588-95. Species: Primate - Baboon Sample Type: Tissue Homogenates Application: WB Activation of hypoxia-inducible factors in hyperoxia through prolyl 4-hydroxylase blockade in cells and explants of primate lung. Authors: Asikainen TM, Schneider BK, Waleh NS, Clyman RI, Ho WB, Flippin LA, Gunzler V, White CW Proc. Natl. Acad. Sci. U.S.A., 2005;102(29):10212-7. Species: Human Sample Type: Whole Cells Application: ICC PEDF derived from glial Muller cells: a possible regulator of retinal angiogenesis. Authors: Eichler W, Yafai Y, Keller T, Wiedemann P, Reichenbach A Exp. Cell Res., 2004;299(1):68-78. Species: Human Sample Type: Whole Cells Application: Neut VEGF164(165) as the pathological isoform: differential leukocyte and endothelial responses through VEGFR1 and VEGFR2. Authors: Usui T, Ishida S, Yamashiro K, Kaji Y, Poulaki V, Moore J, Moore T, Amano S, Horikawa Y, Dartt D, Golding M, Shima DT, Adamis AP Invest. Ophthalmol. Vis. Sci., 2004;45(2):368-74. Species: Human Sample Type: Whole Cells Application: Neut Malignant mesothelioma growth inhibition by agents that target the VEGF and VEGF-C autocrine loops. Authors: Masood R, Kundra A, Zhu S, Xia G, Scalia P, Smith DL, Gill PS Int. J. Cancer, 2003;104(5):603-10. Species: Human Sample Type: Cell Lysates Application: WB Proangiogenic properties of human myeloma cells: production of angiopoietin-1 and its potential relationship to myeloma-induced angiogenesis. Authors: Giuliani N, Colla S, Lazzaretti M, Sala R, Roti G, Mancini C, Bonomini S, Lunghi P, Hojden M, Genestreti G, Svaldi M, Coser P, Fattori PP, Sammarelli G, Gazzola GC, Almici C, Caramatti C, Mangoni L, Rizzoli V Blood, 2003;102(2):638-45. Species: Human Sample Type: Whole Cells Application: Neut Human herpesvirus 8 (HHV-8)-encoded cytokines induce expression of and autocrine signaling by vascular endothelial growth factor (VEGF) in HHV-8-infected primary-effusion lymphoma cell lines and mediate VEGF-independent antiapoptotic effects. Authors: Okruzhnov Y, Nicholas J J. Virol., 2001;75(22):10933-40. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human VEGF R2/KDR/Flk-1 Ala20-Glu764 Accession # AAC16450
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	VEGF R2 (KDR/Flk-1), VEGF R1 (Flt-1) and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGF R1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. VEGF R2 cDNA encodes a 1356 amino acid (aa) residue precursor protein with a 19 aa residue signal peptide. Mature VEGF R2 is composed of a 745 aa residue extracellular domain, a 25 aa residue transmembrane domain and a 567 aa residue cytoplasmic domain. In contrast to VEGF R1 which binds both PlGF and VEGF with high affinity, VEGF R2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGF R2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.
运输条件	Blue Ice
存放说明	4℃
参考文献	Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.

参考图片

Detection of Human VEGF R2/KDR/Flk‑1 by Western Blot. Western blot shows lysate of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human VEGF R2/KDR/Flk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF357) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for VEGF R2/KDR/Flk‑1 at approximately
200-250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

VEGF R2/KDR/Flk‑1 in Human Placenta. VEGF R2/KDR/Flk‑1 was detected in immersion fixed paraffin-embedded sections of human placenta using 15 µg/mL Goat Anti-Human VEGF R2/
KDR/Flk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF357) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

VEGF R2/KDR/Flk‑1 in Human Kidney. VEGF R2/KDR/Flk‑1 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human VEGF R2/KDR/Flk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF357) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

VEGF R2/KDR/Flk‑1 Inhibition of VEGF-dependent Cell Proliferation and Neutralization by Human VEGF R2/KDR/Flk‑1 Antibody. Recombinant Human VEGF R2/KDR/Flk‑1 Fc Chimera (Catalog # 357-KD) inhibits Recombinant Human VEGF₁₆₅(Catalog #293-VE) induced proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Inhibition of Recombinant Human VEGF₁₆₅ (5 ng/mL) activity elicited by Recombinant Human VEGF R2/KDR/Flk‑1 Fc Chimera (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human VEGF R2/KDR/Flk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF357). The ND₅₀ is typically
0.05‑0.25 µg/mL.