Human ASK1 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human ASK1 Affinity Purified Polyclonal Ab (25 UG)

货号： AF3575-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF3575-SP
别名	Apoptosis signal-regulating kinase 1; ASK-1; ASK1MEKK 5; EC 2.7.11; MAP/ERK kinase kinase 5; MAP3K5; MAPK/ERK kinase kinase 5; MAPKKK5EC 2.7.11.25; MEK kinase 5; MEKK5; MEKK5apoptosis signal regulating kinase 1; mitogen-activated protein kinase kinase kinase 5
全称	Apoptosis Signal-regulated Kinase 1
反应种属	Human
应用	Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects human ASK1 in direct ELISAs and Western blots.
使用方法	Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4217 (Human); 26408 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Semaphorin7A regulates neuroglial plasticity in the adult hypothalamic median eminence. Authors: Parkash J, Messina A, Langlet F, Cimino I, Loyens A, Mazur D, Gallet S, Balland E, Malone S, Pralong F, Cagnoni G, Schellino R, De Marchis S, Mazzone M, Pasterkamp R, Tamagnone L, Prevot V, Giacobini P Nat Commun, 2015;6(0):6385. Pre-exposure of Mycobacterium tuberculosis-infected macrophages to crystalline silica impairs control of bacterial growth by deregulating the balance between apoptosis and necrosis. Authors: Chavez-Galan L, Ramon-Luing L, Torre-Bouscoulet L, Perez-Padilla R, Sada-Ovalle I PLoS ONE, 2013;8(11):e80971. Species: Human Sample Type: Cell Lysate Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human ASK1 Lys1011-Asp1196 Accession # Q99683
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Apoptosis signal-regulating kinase 1 (ASK1) is a MAP kinase kinase kinase also known as MEKK5 and MAP3K5. ASK1 is induced by inflammatory cytokines, UV light, and other stress-related stimuli to phosphorylate MAP kinase kinases that in turn activate the p38 and JNK families of MAP kinases. Mice lacking ASK1 are resistant to stress-induced p38 and JNK activation and cell death.
运输条件	Blue Ice
存放说明	4℃
参考文献	Apoptosis Intracellular Kinases Intracellular Kinases Intracellular Kinases in the Akt Pathway TNF Superfamily Regulators

参考图片

Detection of Human ASK1 by Western Blot. Western blot shows lysates of Raji human Burkitts lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ASK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3575) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ASK1 at approximately 154 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.