| 货号 | AF3556-SP |
| 别名 | Apo B-100; APOB; apoB-100; apoB-48; apolipoprotein B (including Ag(x) antigen); apolipoprotein B-100; apolipoprotein B48; FLDB; LDLCQ4; mutant Apo B 100 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(1-15 µg/mL) |
| 目标/特异性 | Detects human Apolipoprotein B/ApoB in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 1-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 338 (Human); 238055 (Mouse); 54225 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Filter-Dense Multicolor Microscopy. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Apolipoprotein B/ApoB Met1206-Asp1413 Accession # NP_000375 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | ApoB is a component of LDL and VLDL particles and chylomicans. ApoB100, the full-length ApoB synthesized in the liver, is a 512 kDa secreted palmetoylated glycoprotein that contains 4536 amino acid residues. A truncated ApoB designated ApoB48, containing the N-terminal 48% of ApoB100, is also produced by the intestine as a result of post-transcriptional modification of the ApoB mRNA. Additionally, C-terminal truncated ApoB, varying in length from ApoB25 to ApoB89, exists as a result of mutations in the ApoB gene. Within the region used as the immunogen, the amino acid sequence of the human peptide is 80% and 82% identical to the mouse and canine sequence, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Apolipoprotein B/ApoB by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Apolipoprotein B/ApoB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3556) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Apolipoprotein B/ApoB at approximately 500 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Apolipoprotein B/ApoB in Human Liver. Apolipoprotein B/ApoB was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human Apolipoprotein B/ApoB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3556) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm and plasma membrane of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
