| 货号 | AF3549-SP |
| 别名 | DISPA; DISPAFLJ43740; dispatched A; dispatched homolog 1 (Drosophila); Dispatched; DKFZp434I0428; MGC104180; MGC13130; MGC16796; protein dispatched homolog 1 | 全称 | Dispatched 1 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human DISP1 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 84976 (Human); 68897 (Mouse); 289338 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human DISP1 Glu212-Lys492, Ser746-Thr984 Accession # NP_116279 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | DISP1 is a multispan (12) transmembrane protein with cytoplasmic N- and C-terminal tails. It contains a stero/sensing domain (SSD) and has been shown to play a role in regulating the release of cholesterol-modified SHH. Within the regions used for immunization, human and mouse DISP1 share 93% amino acid sequence homology. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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DISP1 in BG01V Human Embryonic Stem Cells. DISP1 was detected in immersion fixed BG01V human embryonic stem cells using Sheep Anti-Human DISP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3549) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
