| 货号 | AF3544-SP |
| 别名 | annexin 14; annexin A10; annexin-10; annexin-14; ANX14; ANX14Annexin-10; ANXA10 |
| 反应种属 | Human |
| 应用 | Western Blot(0.5 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects endogenous human Annexin A10 in Western blots. In Western blots, this antibody does not cross-react with recombinant human Annexin A1, A2, A3, A4, A6, A7, A8, A9, A11, or A13. |
| 使用方法 | Western Blot: 0.5 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 11199 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Annexin A10 Met1-Tyr324 Accession # Q9UJ72 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The Annexins are a family of Calcium-dependent phospholipid-binding proteins that are preferentially located on the cytosolic face of the plasma membrane. The Annexin’s have a molecular weight of approximately 35 to 40 kDa and consist of a unique amino terminal domain followed by a homologous C-terminal core domain containing the calcium-dependent phospholipid-binding sites. The C‑terminal domain is comprised of four 60‑70 amino acid repeats, known as annexin repeats or an endonexin fold (Annexin A6 contains 8 annexin repeats). The four annexin repeats form a highly alpha -helical, tightly packed disc known as the annexin domain, which binds to phospholipids in the membrane in a calcium-dependent manner. Members of the annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth, and intracellular signal transduction pathways. Annexin A10 (ANXA10), also known as Annexin 14, is a 37 kDa member of the Annexin family of proteins. The function of Annexin A10 has not been clearly defined. Human Annexin A10 shares 89% identity with mouse Annexin A10. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Annexin A10 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Human Annexin A10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3544) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Annexin A10 at approximately 37 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2. | |
Annexin A10 in Human Stomach. Annexin A10 was detected in immersion fixed paraffin-embedded sections of human stomach using Goat Anti-Human Annexin A10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3544) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
