| 货号 | AF352-SP |
| 别名 | D3F15S2; EC 3.4.21; hepatocyte growth factor-like protein homolog; HGFL; HGFLhepatocyte growth factor-like protein; macrophage stimulating 1 (hepatocyte growth factor-like); Macrophage stimulatory protein; Macrophage-stimulating protein; MSPDNF15S2; MST1; NF15S2; SF2 | 全称 | Macrophage Stimulating Protein/Macrophage Stimulating 1 [Hepatocyte Growth Factor-like] |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects human MSP/MST1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human HGF is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize MSP-induced chemotaxis in mouse peritoneal resident macrophages. Ming-Hai, W. et al. (1994) J. Biol. Chem. 269:3436‑3440. The Neutralization Dose (ND50) is typically 0.05-0.2 µg/mL in the presence of 50 ng/mL Recombinant Human MSP. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4485 (Human); 15235 (Mouse); 24566 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Macrophage-stimulating protein is produced by tubular cells and activates mesangial cells. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human MSP/MST1 Gln19-Gly711 Accession # AAA59872 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Macrophage stimulating protein (MSP), also known as HGF-like protein, and scatter factor-2, is a member of the HGF family of growth factors (1). MSP is secreted as an inactive single chain precursor (pro-MSP) that contains a PAN/APPLE-like domain, four kringle domains, and a peptidase S1 domain which lacks enzymatic activity (2). Human MSP shares 79% aa sequence identity with mouse MSP and 44% aa sequence identity with human HGF. Pro-MSP is secreted by hepatocytes under the positive and negative control of CBP in complex with either HNF-4 or RAR, respectively (3). Circulating pro-MSP is proteolytically cleaved in response to tissue injury to yield biologically active disulfide linked heterodimers consisting of a 45‑62 kDa alpha and a 25‑35 kDa beta chain (4, 5). Pro-MSP can be activated by MT-SP1, a transmembrane protease that is expressed on macrophages and is upregulated in many cancers (6). Heterodimeric MSP as well as the isolated beta chain bind to MSP R/Ron with high-affinity, although only heterodimeric MSP can induce receptor dimerization and signaling (7, 8). MSP induces macrophage and keratinocyte proliferation and osteoclast activation (9, 10). It also inhibits LPS- or IFN-induced iNOS and IL-12 expression by macrophages and prevents apoptosis of epithelial cells separated from the ECM (11, 12). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Chemotaxis Induced by MSP and Neutralization by Human MSP Antibody. Recombinant Human MSP (Catalog # 352‑MS) chemoattracts mouse peritoneal resident macrophages in a dose-dependent manner (orange line). The amount of cells that migrated through the filter was measured by LeukoStat™ staining (Fisher Scientific). Chemotaxis elicited by Recombinant Human MSP (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human MSP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF352). The ND50 is typically 0.05‑0.2 µg/mL. |
