Human VEGF R3/Flt-4 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human VEGF R3/Flt-4 Affinity Purified Polyclonal Ab (25 UG)

货号： AF349-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF349-SP
别名	EC 2.7.10; EC 2.7.10.1; FLT4; Flt-4; fms-related tyrosine kinase 4; LMPH1A; PCLFLT41; soluble VEGFR3 variant 1; soluble VEGFR3 variant 2; soluble VEGFR3 variant 3; Tyrosine-protein kinase receptor FLT4; vascular endothelial growth factor receptor 3; VEGFR-3; VEGFR3Fms-like tyrosine kinase 4
全称	Vascular Endothelial Growth Factor Receptor 3
反应种属	Human
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human VEGF R3 in direct ELISAs and Western blots. In Western blots, approximately 15% cross-reactivity with recombinant mouse VEGF R3 is observed and less than 2% cross-reactivity with recombinant human VEGF R1 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2324 (Human); 14257 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Primary Tumor-Secreted Lymphangiogenic Factors Induce Pre-Metastatic Lymphvascular Niche Formation at Sentinel Lymph Nodes in Oral Squamous Cell Carcinoma. Authors: Wakisaka N, Hasegawa Y, Yoshimoto S, Miura K, Shiotani A, Yokoyama J, Sugasawa M, Moriyama-Kita M, Endo K, Yoshizaki T PLoS ONE, 2015;10(12):e0144056. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded VEGF-D promotes tumor growth and lymphatic spread in a mouse model of hepatocellular carcinoma. Authors: Thelen A, Scholz A, Benckert C, Von Marschall Z, Schroder M, Wiedenmann B, Neuhaus P, Rosewicz S, Jonas S Int. J. Cancer, 2008;122(11):2471-81. Species: Human Sample Type: Whole Tissue Application: IHC Cyclooxygenase-2 induces EP1- and HER-2/Neu-dependent vascular endothelial growth factor-C up-regulation: a novel mechanism of lymphangiogenesis in lung adenocarcinoma. Authors: Su JL, Shih JY, Yen ML, Jeng YM, Chang CC, Hsieh CY, Wei LH, Yang PC, Kuo ML Cancer Res., 2004;64(2):554-64. Species: Human Sample Type: Cell Lysates Application: WB All three receptors for vascular endothelial growth factor (VEGF) are expressed on B-chronic lymphocytic leukemia (CLL) cells. Authors: Bairey O, Boycov O, Kaganovsky E, Zimra Y, Shaklai M, Rabizadeh E Leuk. Res., 2004;28(3):243-8. Species: Human Sample Type: Whole Cells Application: ICC Expression of vascular endothelial growth factor (VEGF)-D and its receptor, VEGF receptor 3, as a prognostic factor in endometrial carcinoma. Authors: Yokoyama Y, Charnock-Jones DS, Licence D, Yanaihara A, Hastings JM, Holland CM, Emoto M, Sakamoto A, Sakamoto T, Maruyama H, Sato S, Mizunuma H, Smith SK Clin. Cancer Res., 2003;9(4):1361-9. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Malignant mesothelioma growth inhibition by agents that target the VEGF and VEGF-C autocrine loops. Authors: Masood R, Kundra A, Zhu S, Xia G, Scalia P, Smith DL, Gill PS Int. J. Cancer, 2003;104(5):603-10. Species: Human Sample Type: Cell Lysates Application: IP
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human VEGF R3 Tyr25-Ile776 Accession # P35916
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	VEGF R2 (KDR/Flk-1), VEGF R1 (Flt-1) and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGF R1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. VEGF R3 cDNA encodes a 1298 amino acid (aa) residue precursor protein with a 24 aa residue signal peptide. Mature VEGF R3 is composed of a 751 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 482 aa residue cytoplasmic domain. Both VEGF-C and VEGF-D have been shown to bind and activate VEGF R3 (Flt-4). VEGF R3 is widely expressed in the early embryo but becomes restricted to lymphatic endothelia at later stages of development. It is likely that VEGF R3 may be important for lymph angiogenesis.
运输条件	Blue Ice
存放说明	4℃
参考文献	Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.

参考图片

VEGF R3/Flt‑4 in Human Uterine Cancer Tissue. VEGF R3/Flt‑4 was detected in immersion fixed paraffin-embedded sections of human uterine cancer tissue using Human VEGF R3/Flt‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF349) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.