| 货号 | AF3480-SP |
| 别名 | CLAXLLT-1; CLEC2D; C-type lectin domain family 2 member D; C-type lectin domain family 2, member D; C-type lectin related f; Lectin-like NK cell receptor; Lectin-like transcript 1; LLT1; LLT1Osteoclast inhibitory lectin; OCILmember D | 全称 | Osteoclast Inhibitory Lectin |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/106cells) |
| 目标/特异性 | Detects human OCIL/CLEC2d in direct ELISAs and Western blots. In Western blots, approximately 15% cross-reactivity with recombinant mouse (rm) OCIL is observed and less than 1% cross-reactivity with rmOCILrp2, recombinant human (rh) CLEC4D, and rhCLEC4F is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/106cells |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 29121 (Human); 93694 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Expression of lectin-like transcript-1 in human tissues | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human OCIL/CLEC2d Ser57-Val191 Accession # Q9UHP7 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Human OCIL/CLEC2d, also known as Lectin-Like Transcript-1 (LLT-1), is a type II transmembrane protein belonging to the natural killer (NK) cell receptor group of the C-type lectin superfamily. It is found on hematopoietic cells, osteoblasts, and chondrocytes. By alternative splicing, at least three isoforms exist. Isoform 1 is synthesized as a 191 amino acid (aa) precursor with a C-terminal 132 aa extracellular domain (ECD) that contains the C-type lectin domain. The aa sequence of human OCIL isoform 1 ECD is 49% and 50% identical to the mouse and rat OCIL ECD, respectively. Human OCIL preferentially binds high molecular weight sulfated glycosaminoglycans and is a ligand for the single human NKR-P1A receptor (CD161). Human OCIL blocks osteoclast differentiation. Engagement of OCIL with CD161 on NK cells also inhibits NK cell-mediated cytotoxicity and IFN-gamma secretion. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of OCIL/CLEC2d in Raji Human Cell Line by Flow Cytometry. Raji human Burkitts lymphoma cell line was stained with Goat Anti-Human OCIL/CLEC2d Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3480, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). |
