Human Wnt-7b Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Human Wnt-7b Affinity Purified Polyclonal Ab (25 UG)

货号： AF3460-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF3460-SP
别名	protein Wnt-7b; wingless-type MMTV integration site family, member 7B
全称	Wingless-type MMTV Integration Site Family, Member 7B
反应种属	Human
应用	Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human Wnt-7b in direct ELISAs and Western blots. In Western blots, approximately 20% cross-reactivity with recombinant human (rh) Wnt-7a is observed and less than 5% cross-reactivity with recombinant mouse (rm) Wnt-1, rmWnt-3a, rmWnt-4, rmWnt-5a, rhWnt‑9a, rmWnt-11, and rmWnt-16 is observed.
使用方法	Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7477 (Human); 22422 (Mouse); 315196 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Downregulated miR329 and miR410 promote the proliferation and invasion of oral squamous cell carcinoma by targeting Wnt-7b. Authors: Shiah S, Hsiao J, Chang W, Chen Y, Jin Y, Wong T, Huang J, Tsai S, Hsu Y, Chou S, Yen Y, Jiang S, Shieh Y, Chang I, Hsiao M, Chang J Cancer Res, 2014;74(24):7560-72. Species: Human Sample Type: Whole Tissue Application: IHC CD82 inhibits canonical Wnt signalling by controlling the cellular distribution of beta-catenin in carcinoma cells. Authors: Chigita S, Sugiura T, Abe M, Kobayashi Y, Shimoda M, Onoda M, Shirasuna K Int J Oncol, 2012;41(6):2021-8. Species: Human Sample Type: Cell Lysates Application: WB Parabronchial smooth muscle constitutes an airway epithelial stem cell niche in the mouse lung after injury. Authors: Volckaert T, Dill E, Campbell A, Tiozzo C, Majka S, Bellusci S, De Langhe SP J. Clin. Invest., 2011;121(11):4409-19. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Wnt signaling mediates experience-related regulation of synapse numbers and mossy fiber connectivities in the adult hippocampus. Authors: Gogolla N, Galimberti I, Deguchi Y, Caroni P Neuron, 2009;62(4):510-25. Species: Mouse Sample Type: Tissue Homogenates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human Wnt‑7b Leu32-Glu72 & Thr216-Ala283 Accession # P56706
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Wnt-7b is a 46 kDa, secreted glycosylated protein that belongs to theWnt family. Wnt proteins can be lipid-modified and are ligands formembers of the frizzled family of receptors, which mediates cell-cellcommunication during development. Human Wnt-7b is synthesized as a 349amino acid (aa) precursor that contains a 318 aa mature region. Themature region contains 24 cysteines and three potential N-linkedglycosylation sites. Mature human Wnt-7b shares 99% aa sequenceidentity with mature mouse and rat Wnt-7b. Wnt-7b also shares 80% aasequence identity with Wnt-7a.
运输条件	Blue Ice
存放说明	4℃
参考文献	A Leader in the Development of Reagents for Wnt Research Wnt Ligands (Wnts)

参考图片

Detection of Human Wnt‑7b by Western Blot. Western blot shows lysates of JAR human choriocarcinoma cell line and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Wnt‑7b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3460) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Wnt‑7b at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.