| 货号 | AF3456-SP |
| 别名 | C1q and tumor necrosis factor related protein 4; complement C1q tumor necrosis factor-related protein 4; complement-c1q tumor necrosis factor-related protein 4; CTRP4; CTRP4ZACRP4; ZACRP4 | 全称 | C1q And Tumor Necrosis Factor Related Protein 4 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Intracellular Staining by Flow Cytometry(0.25 µg/106cells) |
| 目标/特异性 | Detects human C1qTNF4 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) C1qTNF5 is observed and less than 1% cross-reactivity with rhC1qTNF1 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Intracellular Staining by Flow Cytometry: 0.25 µg/106cells |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 114900 (Human); 67445 (Mouse); 311184 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human C1qTNF4 Leu17-Leu329 Accession # Q9BXJ3 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Cq1TNF4, also known as CTRP4, is a secreted protein and is one of at least 9 paralogs of Acrp30/Adiponectin. Proteins in this family contain a globular C-terminal domain similar to that of the C1q and TNF ligand family. C1qTNF4 is the only family member that lacks the collagen-like domain but has two C1q domains. Mature human C1qTNF4 shares 92.4% amino acid sequence homology with the corresponding region of mouse C1qTNF4. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of C1qTNF4 in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Goat Anti-Human C1qTNF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3456, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog #FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
