| 货号 | AF3450-SP |
| 别名 | GLC10; GLC1O; neurotrophic factor 4; neurotrophic factor 5; neurotrophin 4; neurotrophin 5 (neurotrophin 4/5); neurotrophin-4; neurotrophin-5; Neutrophic factor 4; NT4; NT-4; NT-4/5; NT-5; NTF4; NTF5; NTF5NT5 | 全称 | Neurotrophin 4 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human NT-4 Propeptide in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with mature recombinant human NT-4 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4909 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human NT‑4 Propeptide Gln25-Arg80 Accession # P34130 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | NT-4 Propeptide (neurotrophin-4/5) is a 27 kDa, secreted, glycosylated neuronal polypeptide that belongs to the NGF-beta family of neurotrophic factors. It is synthesized as a proform that contains a 9 kDa, 56 amino acid (aa) proregion (aa 25-80) and a 130 aa mature segment. The mature molecule forms both nondisulfide linked heterodimers (with NGF) and homodimers. It is unclear if 186 aa NT-4 Propeptide dimerizes, but this might be anticipated. Proneurotrophins are believed to activate NGFR and sortilin complexes and neurotrophin proregions regulate neurotrophin maturation. Over aa 25-80, human NT-4 Propeptide is 89% and 91% aa identical to mouse and canine NT-4 Propeptide, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human NT‑4 Propeptide by Western Blot. Western blot shows lysates of PC‑3 human prostate cancer cell line, LNCaP human prostate cancer cell line, and DU145 human prostate carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human NT‑4 Propeptide Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3450) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for NT‑4 Propeptide at approximately 36 kDa (corresponent to proform). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
