| 货号 | AF3444-SP |
| 别名 | Atoh5; BHLHA7; bHLHa7NGN-3; Class A basic helix-loop-helix protein 7; Math4B; NEUROG3; neurogenin 3; neurogenin-3; NGN3; Protein atonal homolog 5; Relax |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human Neurogenin-3 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse Neurogenin-3 is observed, and less than 1% cross-reactivity with recombinant human Neurogenin-1 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 50674 (Human); 11925 (Mouse); 60329 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The SDF-1α/CXCR4 Axis is Required for Proliferation and Maturation of Human Fetal Pancreatic Endocrine Progenitor Cells. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Neurogenin-3 Met1-Leu214 Accession # Q9Y4Z2 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Neurogenin-3 (NGN3; also Protein atonal homolog 5) is a 23 kDa, developmentally expressed nuclear protein that belongs to a family of atonal-related basic helix‑loop‑helix (bHLH) transcription factors. bHLH proteins typically form dimers when binding to DNA. NGN3 expression is required for the lineage differentiation of all endocrine cell types in the pancreas, including the formation of islet beta cells. NGN3 is also neurogenic, controlling the number of dendrites generated by hippocampal neurons. Human NGN3 is 214 amino acids (aa) in length. It contains a DNA binding region (aa 84‑95) plus an HLH motif (aa 96‑136). Over aa 3‑214, human NGN3 shares 76% and 84% aa identity with mouse and canine NGN3, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Neurogenin‑3 by Western Blot. Western blot shows lysates of human placenta and prostate tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Neurogenin‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3444) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Neurogenin‑3 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
