| 货号 | AF3430-SP |
| 别名 | EC 3.1.3.48; protein tyrosine phosphatase PTPsigma; protein tyrosine phosphatase, receptor type, S; protein tyrosine phosphatase, receptor type, sigma; PTPR sigma; PTPRS; PTPSIGMA; receptor-type tyrosine-protein phosphatase S; Receptor-type tyrosine-protein phosphatase sigma; R-PTP-S; R-PTP-sigma | 全称 | Protein Tyrosine Phosphatase Receptor-type Sigma |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects the endogenous cleaved extracellular domain of human PTP sigma /PTPRS in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human LAR, PTPR beta, PTPRδ, PTPRµ, or PTPR kappa is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5802 (Human); 19280 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Modulation of the proteoglycan receptor PTPsigma promotes recovery after spinal cord injury. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human PTP sigma /PTPRS Glu30-Gly1260 Accession # NP_570924 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Protein Tyrosine Phoshatase Receptor-type sigma (PTPR sigma ), also known as PTP sigma, is an integral membrane protein with a non-glycosylated molecular weight of 214 kilodaltons. The extracellular domain, which was used as immunogen, is cleaved near the cell membrane by an endoprotease to generate a glycated 140 kDa protein. Significant levels of this protein are found in the brain and in endocrine tissues such as the pituitary gland and pancreas. Knockout mice deficient in PTPR sigma have severe neurological deficits and most die shortly after birth. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human PTP sigma /PTPRS by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, CHP-100 human neuroblastoma cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human PTP sigma /PTPRS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3430) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human PTPRµ, PTPR kappa, LAR, PTPR sigma, PTPRδ, and PTPR beta (40 ng/lane) were included. A specific band for PTP sigma /PTPRS was detected at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
PTP sigma /PTPRS in A431 Cell Line. PTP sigma /PTPRS was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Human PTP sigma /PTPRS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3430) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
