| 货号 | AF3428-SP |
| 别名 | EC 3.1.3.48; Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP; Lymphoid phosphatase; lymphoid-specific protein tyrosine phosphatase; LYP; Lyp1; Lyp2; LyPTP; PEP; PEST-domain phosphatase; protein tyrosine phosphatase, non-receptor type 22 (lymphoid); protein tyrosine phosphatase, non-receptor type 8; PTPN22; PTPN8 (former); PTPN8LYP1; tyrosine-protein phosphatase non-receptor type 22 | 全称 | Lymphoid Phosphatase |
| 反应种属 | Human |
| 应用 | Western Blot(0.3 µg/mL) Simple Western(3 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Lyp in Western blots. |
| 使用方法 | Western Blot: 0.3 µg/mL Simple Western: 3 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 26191 (Human); 19260 (Mouse); 295338 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. PTPN22.6, a dominant negative isoform of PTPN22 and potential biomarker of rheumatoid arthritis. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Lyp Ser306-Ser684 Accession # Q9Y2R2 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Lymphoid phosphatase (Lyp), also called LyPTP, Protein Tyrosine Phosphatase, Nonreceptor-type 22 (PTPN22), and PEST-domain Phosphatase (PEP), dephosphorylates tyrosine residues in proteins. Found primarily in bone marrow and lymphoid cell lines, an R620W gain-of-function mutation in Lyp has been associated with susceptibility to autoimmune diseases such as type I diabetes, rheumatoid arthritis, and lupus erythematosus. Mature PEP knockout mice have an excess of CD8 positive T-cells and an exaggerated antigen-induced proliferative response. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human Lyp by Western Blot. Western blot shows lysates of Daudi human Burkitts lymphoma cell line and Ramos human Burkitts lymphoma cell lines. PVDF membrane was probed with 0.3 µg/mL of Goat Anti-Human Lyp Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3428) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Lyp at approximately 108 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Lyp in Human PBMCs. Lyp was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human Lyp Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3428) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. | |
Detection of Human Lyp by Simple WesternTM. Simple Western lane view shows lysates of Daudi human Burkitts lymphoma cell line and Ramos human Burkitts lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Lyp at approximately 116-118 kDa (as indicated) using 3 µg/mL of Goat Anti-Human Lyp Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3428) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system. |
